TY - JOUR
T1 - Expression patterns of cell cycle and apoptosis-related genes in a multidrug-resistant human colon carcinoma cell line
AU - Fan, C. W.
AU - Chan, C. C.
AU - Chao, C. C.K.
AU - Fan, H. A.
AU - Sheu, D. L.
AU - Chan, E. C.
PY - 2004/5
Y1 - 2004/5
N2 - Background: An in vitro multidrug resistance (MDR) system from a human colonic cancer cell line (SW620-MDR) has been established. To further study the mechanisms at molecular level and prevention of multidrug resistance in clinical practice, it was demonstrated that the expressions of several apoptosis-related and cell cycle regulator genes were changed in the cells. Methods: A multidrug-resistant colonic cell line (SW620-MDR) was established, and the Atlas human cDNA expression array was used for studying the pattern of gene expression in this cell line. Furthermore, Northern hybridization or real-time PCR analysis confirmed the pattern of gene expression. Results: In the SW620-MDR cell line the proapoptosis genes. CASP4, BIK, PDCD2, and TACE were expressed with decreased levels, and the anti-apoptosis genes CD27-L and IGFBP2 were over-expressed. Furthermore, the cell cycle regulator genes such as CDK6, CCND1, CDC27HS, CDC16HS, Wee1Hu, MAPKK1 and IGFBP6 were expressed with decreased levels in the drug-resistant cell line. Conclusions: It is worthwhile investigating whether the differentially expressed pattern of the aforementioned genes exists in the drug-resistant cancer specimens, and to further understand their functions in the cancer drug-resistance mechanism.
AB - Background: An in vitro multidrug resistance (MDR) system from a human colonic cancer cell line (SW620-MDR) has been established. To further study the mechanisms at molecular level and prevention of multidrug resistance in clinical practice, it was demonstrated that the expressions of several apoptosis-related and cell cycle regulator genes were changed in the cells. Methods: A multidrug-resistant colonic cell line (SW620-MDR) was established, and the Atlas human cDNA expression array was used for studying the pattern of gene expression in this cell line. Furthermore, Northern hybridization or real-time PCR analysis confirmed the pattern of gene expression. Results: In the SW620-MDR cell line the proapoptosis genes. CASP4, BIK, PDCD2, and TACE were expressed with decreased levels, and the anti-apoptosis genes CD27-L and IGFBP2 were over-expressed. Furthermore, the cell cycle regulator genes such as CDK6, CCND1, CDC27HS, CDC16HS, Wee1Hu, MAPKK1 and IGFBP6 were expressed with decreased levels in the drug-resistant cell line. Conclusions: It is worthwhile investigating whether the differentially expressed pattern of the aforementioned genes exists in the drug-resistant cancer specimens, and to further understand their functions in the cancer drug-resistance mechanism.
KW - Apoptosis-related genes
KW - Chemotherapy
KW - cDNA microarray
UR - http://www.scopus.com/inward/record.url?scp=2442570730&partnerID=8YFLogxK
U2 - 10.1080/00365520310008809
DO - 10.1080/00365520310008809
M3 - 文章
C2 - 15180185
AN - SCOPUS:2442570730
SN - 0036-5521
VL - 39
SP - 464
EP - 469
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
IS - 5
ER -
