Extracellular vesicles of adipose‐derived stem cells promote the healing of traumatized achilles tendons

Shih Heng Chen; Zhi Yu Chen; Ya Hsuan Lin; Shih Hsien Chen; Pang Yun Chou; Huang Kai Kao; Feng Huei Lin

doi:10.3390/ijms222212373

Extracellular vesicles of adipose‐derived stem cells promote the healing of traumatized achilles tendons

Shih Heng Chen, Zhi Yu Chen, Ya Hsuan Lin, Shih Hsien Chen, Pang Yun Chou, Huang Kai Kao^*, Feng Huei Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

31 Scopus citations

Abstract

Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesen-chymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose‐derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC‐EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC‐EVs in tendon regeneration, ADSC‐ EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC‐EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC‐EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC‐EV treatment is a potential highly potent therapeutic strategy for tendon injuries.

Original language	English
Article number	12373
Journal	International Journal of Molecular Sciences
Volume	22
Issue number	22
DOIs	https://doi.org/10.3390/ijms222212373
State	Published - 01 11 2021

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Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Adipose‐derived stem cells
Extracellular vesicles
Tendon healing
Tendon regeneration

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@article{10ca87eb41644167a405702b1ecc86b3,

title = "Extracellular vesicles of adipose‐derived stem cells promote the healing of traumatized achilles tendons",

abstract = "Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesen-chymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose‐derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC‐EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC‐EVs in tendon regeneration, ADSC‐ EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC‐EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC‐EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC‐EV treatment is a potential highly potent therapeutic strategy for tendon injuries.",

keywords = "Adipose‐derived stem cells, Extracellular vesicles, Tendon healing, Tendon regeneration",

author = "Chen, {Shih Heng} and Chen, {Zhi Yu} and Lin, {Ya Hsuan} and Chen, {Shih Hsien} and Chou, {Pang Yun} and Kao, {Huang Kai} and Lin, {Feng Huei}",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = nov,

day = "1",

doi = "10.3390/ijms222212373",

language = "英语",

volume = "22",

journal = "International Journal of Molecular Sciences",

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T1 - Extracellular vesicles of adipose‐derived stem cells promote the healing of traumatized achilles tendons

AU - Chen, Shih Heng

AU - Chen, Zhi Yu

AU - Lin, Ya Hsuan

AU - Chen, Shih Hsien

AU - Chou, Pang Yun

AU - Kao, Huang Kai

AU - Lin, Feng Huei

PY - 2021/11/1

Y1 - 2021/11/1

N2 - Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesen-chymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose‐derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC‐EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC‐EVs in tendon regeneration, ADSC‐ EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC‐EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC‐EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC‐EV treatment is a potential highly potent therapeutic strategy for tendon injuries.

AB - Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesen-chymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose‐derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC‐EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC‐EVs in tendon regeneration, ADSC‐ EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC‐EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC‐EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC‐EV treatment is a potential highly potent therapeutic strategy for tendon injuries.

KW - Adipose‐derived stem cells

KW - Extracellular vesicles

KW - Tendon healing

KW - Tendon regeneration

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DO - 10.3390/ijms222212373

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SN - 1661-6596

VL - 22

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

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ER -

Extracellular vesicles of adipose‐derived stem cells promote the healing of traumatized achilles tendons

Abstract

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Keywords

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