Extracorporeal shock wave effectively attenuates brain infarct volume and improves neurological function in rat after acute ischemic stroke

Chun Man Yuen; Sheng Ying Chung; Tzu Hsien Tsai; Pei Hsun Sung; Tien Hung Huang; Yi Ling Chen; Yung Lung Chen; Han Tan Chai; Yen Yi Zhen; Meng Wei Chang; Ching Jen Wang; Hsueh Wen Chang; Cheuk Kwan Sun; Hon Kan Yip

Extracorporeal shock wave effectively attenuates brain infarct volume and improves neurological function in rat after acute ischemic stroke

Chun Man Yuen, Sheng Ying Chung, Tzu Hsien Tsai, Pei Hsun Sung, Tien Hung Huang, Yi Ling Chen, Yung Lung Chen, Han Tan Chai, Yen Yi Zhen, Meng Wei Chang, Ching Jen Wang, Hsueh Wen Chang, Cheuk Kwan Sun, Hon Kan Yip^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

35 Scopus citations

Abstract

Background: To investigate the effect of shock wave (SW) on brain-infarction volume (BIV) and neurological function in acute ischemic stroke (AIS) by left internal carotid artery occlusion in rats. Methods and results: SD rats (n=48) were divided into group 1 [sham-control (SC)], group 2 [SC-ECSW (energy dosage of 0.15 mJ/mm2/300 impulses)], group 3 (AIS), and group 4 (AIS-ECSW) and sacrificed by day 28 after IS induction. In normal rats, caspase- 3, Bax and TNF-α biomarkers did not differ between animals with and without ECSW therapy, whereas Hsp70 was activated post-ECSW treatment. By day 21 after AIS, Sensorimotor-functional test identified a higher frequency of turning movement to left in group 3 than that in group 4 (P<0.05). By day 28, brain MRI demonstrated lager BIV in group 3 than that in group 4 (P<0.001). Angiogenesis biomarkers at cellular (CD31, α-SMA+) and protein (eNOS) levels and number of neuN+ cells were higher in groups 1 and 2 than those in groups 3 and 4, and higher in group 4 than those in group 3, whereas VEGF and Hsp70 levels were progressively increased from groups 1 and 2 to group 4 (all P<0.001). Protein expressions of apoptosis (Bax, caspase 3, PARP), inflammation (MMP-9, TNF-α), oxidative stress (NOX-1, NOX-2, oxidized protein) and DNA-damage marker (γ-H2AX), and expressions of γ-H2AX+, GFAP+, AQP-4+ cells showed an opposite pattern compared to that of angiogenesis among the four groups (all P<0.001). Conclusion: ECSW therapy was safe and effective in reducing BIV and improved neurological function.

Original language	English
Pages (from-to)	976-994
Number of pages	19
Journal	American Journal of Translational Research
Volume	7
Issue number	6
State	Published - 25 02 2015

Bibliographical note

Publisher Copyright:
© 2015 E-Century Publishing Corporation. All rights reserved.

Keywords

Acute ischemic stroke
Angiogenesis
Extracorporeal shock wave
Inflammation
Oxidative stress

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Yuen, C. M., Chung, S. Y., Tsai, T. H., Sung, P. H., Huang, T. H., Chen, Y. L., Chen, Y. L., Chai, H. T., Zhen, Y. Y., Chang, M. W., Wang, C. J., Chang, H. W., Sun, C. K., & Yip, H. K. (2015). Extracorporeal shock wave effectively attenuates brain infarct volume and improves neurological function in rat after acute ischemic stroke. American Journal of Translational Research, 7(6), 976-994.

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title = "Extracorporeal shock wave effectively attenuates brain infarct volume and improves neurological function in rat after acute ischemic stroke",

abstract = "Background: To investigate the effect of shock wave (SW) on brain-infarction volume (BIV) and neurological function in acute ischemic stroke (AIS) by left internal carotid artery occlusion in rats. Methods and results: SD rats (n=48) were divided into group 1 [sham-control (SC)], group 2 [SC-ECSW (energy dosage of 0.15 mJ/mm2/300 impulses)], group 3 (AIS), and group 4 (AIS-ECSW) and sacrificed by day 28 after IS induction. In normal rats, caspase- 3, Bax and TNF-α biomarkers did not differ between animals with and without ECSW therapy, whereas Hsp70 was activated post-ECSW treatment. By day 21 after AIS, Sensorimotor-functional test identified a higher frequency of turning movement to left in group 3 than that in group 4 (P<0.05). By day 28, brain MRI demonstrated lager BIV in group 3 than that in group 4 (P<0.001). Angiogenesis biomarkers at cellular (CD31, α-SMA+) and protein (eNOS) levels and number of neuN+ cells were higher in groups 1 and 2 than those in groups 3 and 4, and higher in group 4 than those in group 3, whereas VEGF and Hsp70 levels were progressively increased from groups 1 and 2 to group 4 (all P<0.001). Protein expressions of apoptosis (Bax, caspase 3, PARP), inflammation (MMP-9, TNF-α), oxidative stress (NOX-1, NOX-2, oxidized protein) and DNA-damage marker (γ-H2AX), and expressions of γ-H2AX+, GFAP+, AQP-4+ cells showed an opposite pattern compared to that of angiogenesis among the four groups (all P<0.001). Conclusion: ECSW therapy was safe and effective in reducing BIV and improved neurological function.",

keywords = "Acute ischemic stroke, Angiogenesis, Extracorporeal shock wave, Inflammation, Oxidative stress",

author = "Yuen, {Chun Man} and Chung, {Sheng Ying} and Tsai, {Tzu Hsien} and Sung, {Pei Hsun} and Huang, {Tien Hung} and Chen, {Yi Ling} and Chen, {Yung Lung} and Chai, {Han Tan} and Zhen, {Yen Yi} and Chang, {Meng Wei} and Wang, {Ching Jen} and Chang, {Hsueh Wen} and Sun, {Cheuk Kwan} and Yip, {Hon Kan}",

year = "2015",

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day = "25",

language = "英语",

volume = "7",

pages = "976--994",

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TY - JOUR

T1 - Extracorporeal shock wave effectively attenuates brain infarct volume and improves neurological function in rat after acute ischemic stroke

AU - Yuen, Chun Man

AU - Chung, Sheng Ying

AU - Tsai, Tzu Hsien

AU - Sung, Pei Hsun

AU - Huang, Tien Hung

AU - Chen, Yi Ling

AU - Chen, Yung Lung

AU - Chai, Han Tan

AU - Zhen, Yen Yi

AU - Chang, Meng Wei

AU - Wang, Ching Jen

AU - Chang, Hsueh Wen

AU - Sun, Cheuk Kwan

AU - Yip, Hon Kan

PY - 2015/2/25

Y1 - 2015/2/25

N2 - Background: To investigate the effect of shock wave (SW) on brain-infarction volume (BIV) and neurological function in acute ischemic stroke (AIS) by left internal carotid artery occlusion in rats. Methods and results: SD rats (n=48) were divided into group 1 [sham-control (SC)], group 2 [SC-ECSW (energy dosage of 0.15 mJ/mm2/300 impulses)], group 3 (AIS), and group 4 (AIS-ECSW) and sacrificed by day 28 after IS induction. In normal rats, caspase- 3, Bax and TNF-α biomarkers did not differ between animals with and without ECSW therapy, whereas Hsp70 was activated post-ECSW treatment. By day 21 after AIS, Sensorimotor-functional test identified a higher frequency of turning movement to left in group 3 than that in group 4 (P<0.05). By day 28, brain MRI demonstrated lager BIV in group 3 than that in group 4 (P<0.001). Angiogenesis biomarkers at cellular (CD31, α-SMA+) and protein (eNOS) levels and number of neuN+ cells were higher in groups 1 and 2 than those in groups 3 and 4, and higher in group 4 than those in group 3, whereas VEGF and Hsp70 levels were progressively increased from groups 1 and 2 to group 4 (all P<0.001). Protein expressions of apoptosis (Bax, caspase 3, PARP), inflammation (MMP-9, TNF-α), oxidative stress (NOX-1, NOX-2, oxidized protein) and DNA-damage marker (γ-H2AX), and expressions of γ-H2AX+, GFAP+, AQP-4+ cells showed an opposite pattern compared to that of angiogenesis among the four groups (all P<0.001). Conclusion: ECSW therapy was safe and effective in reducing BIV and improved neurological function.

AB - Background: To investigate the effect of shock wave (SW) on brain-infarction volume (BIV) and neurological function in acute ischemic stroke (AIS) by left internal carotid artery occlusion in rats. Methods and results: SD rats (n=48) were divided into group 1 [sham-control (SC)], group 2 [SC-ECSW (energy dosage of 0.15 mJ/mm2/300 impulses)], group 3 (AIS), and group 4 (AIS-ECSW) and sacrificed by day 28 after IS induction. In normal rats, caspase- 3, Bax and TNF-α biomarkers did not differ between animals with and without ECSW therapy, whereas Hsp70 was activated post-ECSW treatment. By day 21 after AIS, Sensorimotor-functional test identified a higher frequency of turning movement to left in group 3 than that in group 4 (P<0.05). By day 28, brain MRI demonstrated lager BIV in group 3 than that in group 4 (P<0.001). Angiogenesis biomarkers at cellular (CD31, α-SMA+) and protein (eNOS) levels and number of neuN+ cells were higher in groups 1 and 2 than those in groups 3 and 4, and higher in group 4 than those in group 3, whereas VEGF and Hsp70 levels were progressively increased from groups 1 and 2 to group 4 (all P<0.001). Protein expressions of apoptosis (Bax, caspase 3, PARP), inflammation (MMP-9, TNF-α), oxidative stress (NOX-1, NOX-2, oxidized protein) and DNA-damage marker (γ-H2AX), and expressions of γ-H2AX+, GFAP+, AQP-4+ cells showed an opposite pattern compared to that of angiogenesis among the four groups (all P<0.001). Conclusion: ECSW therapy was safe and effective in reducing BIV and improved neurological function.

KW - Acute ischemic stroke

KW - Angiogenesis

KW - Extracorporeal shock wave

KW - Inflammation

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=84939511877&partnerID=8YFLogxK

M3 - 文章

AN - SCOPUS:84939511877

SN - 1943-8141

VL - 7

SP - 976

EP - 994

JO - American Journal of Translational Research

JF - American Journal of Translational Research

IS - 6

ER -

Extracorporeal shock wave effectively attenuates brain infarct volume and improves neurological function in rat after acute ischemic stroke

Abstract

Bibliographical note

Keywords

UN SDGs

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