Fabrication and electrochemical characterization of glucose oxidase-immobilized carbon electrode

The synthesis and related electrochemical properties of enzyme, glucose oxidase, modified carbon cloth electrodes, which are mediated by gold nanoparticles (Au NP), are presented. The Au NP with a 4 nm avg dia are used as a significant electron relay or mediator for the alignment of the GO_x on the carbon support. The GO_x is immobilized by flavin adenin dinucleotide cofactor on Au NP. Thus, the biological catalysts, GO_x, become much more stable on conductive carbon supports. Immobilized GO_x can maintain its specific enzyme activity in the presence of glucose. The dependence of current density and the redox potential in the corresponding reaction is examined. As a consequence, under optimal condition, good electrochemical behavior and large current can be achieved, thereby indicating the potential application in biofuel cells. This is an abstract of a paper presented at the ACS Fuel Chemistry Meeting (Washington, DC Fall 2005).