Fabrication and modification of wavy multicomponent vascular grafts with biomimetic mechanical properties, antithrombogenicity, and enhanced endothelial cell affinity

Hao Yang Mi, Xin Jing*, Zhu Tong Li, Yu Jyun Lin, James A. Thomson, Lih Sheng Turng

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

18 Scopus citations

Abstract

A mismatch of mechanical properties and a high rate of thromboses are two critical challenges of creating viable artificial small-diameter vascular grafts (SDVGs). Herein, we propose a method to fabricate wavy multicomponent vascular grafts (WMVGs) via electrospinning using an assembled rotating collector. The WMVGs consisted of a wavy silk/poly(lactic acid) (PLA) inner layer and a thermoplastic polyurethane (TPU) outer layer, which mimic the structures and properties of collagen and elastin in native blood vessels, respectively. Attributed to the wavy structure and the combination of rigid silk/PLA and elastic TPU biomaterials, WMVGs are capable of mimicking the nonlinear tensile stress–strain relationship and “toe region” of native blood vessels. In addition, they have sufficient mechanical strength to meet implantation requirements in terms of tensile strength, suture retention, and burst pressure. Further modification of silk/PLA fibers with dopamine and heparin gave the grafts antithrombogenic properties and greatly enhanced endothelial cell affinities. Human umbilical vein endothelial cells (HUVECs) cultured on modified silk/PLA showed high viability, high proliferation rate, and favorable cell–substrate interactions. Moreover, HUVECs were able to fully cover and freely migrate upward on the lumen of the modified WMVGs without needing a special circulation bioreactor. Therefore, the modified WMVGs possessed biomimetic properties, antithrombogenicity, and enhanced endothelialization, making them a promising candidate for SDVGs.

Original languageEnglish
Pages (from-to)2397-2408
Number of pages12
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume107
Issue number7
DOIs
StatePublished - 01 10 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.

Keywords

  • antithrombosis
  • biomimetic
  • electrospinning
  • vascular grafts
  • vascular tissue engineering

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