Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

Pei Yu Chang; Kuo Wei Chang; Ciou Wen Lin; Po Chen Shih; Cheng Hsien Liu; Wen Syang Hsu; Shih Kang Fan; Chin Jung Li; Chang Hung Tien; Hong Yuan Huang; Da Jeng Yao

doi:10.1109/NANOMED.2012.6509134

Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

Pei Yu Chang, Kuo Wei Chang, Ciou Wen Lin, Po Chen Shih, Cheng Hsien Liu, Wen Syang Hsu, Shih Kang Fan, Chin Jung Li, Chang Hung Tien, Hong Yuan Huang, Da Jeng Yao

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.

Original language	English
Title of host publication	NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
Pages	32-35
Number of pages	4
DOIs	https://doi.org/10.1109/NANOMED.2012.6509134
State	Published - 2012
Externally published	Yes
Event	6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand Duration: 04 11 2012 → 07 11 2012

Publication series

Name	IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN (Print)	2159-6964
ISSN (Electronic)	2159-6972

Conference

Conference	6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
Country/Territory	Thailand
City	Bangkok
Period	04/11/12 → 07/11/12

Keywords

3D chip
Mimetic uterus
Thin membrane

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10.1109/NANOMED.2012.6509134

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Chang, P. Y., Chang, K. W., Lin, C. W., Shih, P. C., Liu, C. H., Hsu, W. S., Fan, S. K., Li, C. J., Tien, C. H., Huang, H. Y., & Yao, D. J. (2012). Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. In NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (pp. 32-35). Article 6509134 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2012.6509134

Chang, Pei Yu ; Chang, Kuo Wei ; Lin, Ciou Wen et al. / Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. pp. 32-35 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED).

@inproceedings{a4333a81ea4a4ad8a635f307e982bbe1,

title = "Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells",

abstract = "Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.",

keywords = "3D chip, Mimetic uterus, Thin membrane",

author = "Chang, {Pei Yu} and Chang, {Kuo Wei} and Lin, {Ciou Wen} and Shih, {Po Chen} and Liu, {Cheng Hsien} and Hsu, {Wen Syang} and Fan, {Shih Kang} and Li, {Chin Jung} and Tien, {Chang Hung} and Huang, {Hong Yuan} and Yao, {Da Jeng}",

year = "2012",

doi = "10.1109/NANOMED.2012.6509134",

language = "英语",

isbn = "9781467351027",

series = "IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED",

pages = "32--35",

booktitle = "NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering",

note = "6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 ; Conference date: 04-11-2012 Through 07-11-2012",

}

Chang, PY, Chang, KW, Lin, CW, Shih, PC, Liu, CH, Hsu, WS, Fan, SK, Li, CJ, Tien, CH, Huang, HY & Yao, DJ 2012, Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. in NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering., 6509134, IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED, pp. 32-35, 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012, Bangkok, Thailand, 04/11/12. https://doi.org/10.1109/NANOMED.2012.6509134

Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. / Chang, Pei Yu; Chang, Kuo Wei; Lin, Ciou Wen et al.
NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. p. 32-35 6509134 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

AU - Chang, Pei Yu

AU - Chang, Kuo Wei

AU - Lin, Ciou Wen

AU - Shih, Po Chen

AU - Liu, Cheng Hsien

AU - Hsu, Wen Syang

AU - Fan, Shih Kang

AU - Li, Chin Jung

AU - Tien, Chang Hung

AU - Huang, Hong Yuan

AU - Yao, Da Jeng

PY - 2012

Y1 - 2012

N2 - Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.

AB - Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.

KW - 3D chip

KW - Mimetic uterus

KW - Thin membrane

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U2 - 10.1109/NANOMED.2012.6509134

DO - 10.1109/NANOMED.2012.6509134

M3 - 会议稿件

AN - SCOPUS:84880194599

SN - 9781467351027

T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED

SP - 32

EP - 35

BT - NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering

T2 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012

Y2 - 4 November 2012 through 7 November 2012

ER -

Chang PY, Chang KW, Lin CW, Shih PC, Liu CH, Hsu WS et al. Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. In NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. p. 32-35. 6509134. (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). doi: 10.1109/NANOMED.2012.6509134

Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

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