Abstract
The human chorionic gonadotropin (hCG) assay for ectopic pregnancy is typically conducted using an enzyme immunoassay (EIA); however, EIA is complicated and time-consuming. Herein, we developed a simple, rapid and sensitive colorimetric assay for hCG based on the anti-aggregation of unmodified gold nanoparticles (AuNPs). The introduction of positively charged hCG-specific peptides into an AuNP solution causes the aggregation of the AuNPs, resulting in a color change from red to blue. When hCG is present, peptide/hCG complexes are formed, thus inhibiting the peptide-induced AuNP aggregation. The dynamic range for hCG detection ranged from 50 to 1000 mIU mL-1, with a detection limit of 25 mIU mL-1. Additionally, this colorimetric sensor was successfully evaluated with serum samples spiked with hCG, demonstrating its ability to be used for the onsite monitoring of hCG.
Original language | English |
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Pages (from-to) | 29-33 |
Number of pages | 5 |
Journal | Analytical Methods |
Volume | 7 |
Issue number | 1 |
DOIs | |
State | Published - 07 01 2015 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2014 The Royal Society of Chemistry.