Facilitating gl13k peptide grafting on polyetheretherketone via 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide: Surface properties and antibacterial activity

In the present work, the antimicrobial peptide (AMP) of GL13K was successfully coated onto a polyetheretherketone (PEEK) substrate to investigate its antibacterial activities against Staphylococcus aureus (S. aureus) bacteria. To improve the coating efficiency, 1‐ethyl‐3‐(3‐dimethyla-minopropyl)carbodiimide (EDC) was mixed with a GL13K solution and coated on the PEEK surface for comparison. Both energy‐dispersive X‐ray spectroscopy (EDX) and X‐ray photoelectron spectroscopy (XPS) data confirmed 30% greater peptide coating on PEEK/GL13K‐EDC than PEEK with-out EDC treatment. The GL13K graft levels are depicted in the micrograms per square centimeter range. The PEEK/GL13K‐EDC sample showed a smoother and lower roughness (Rq of 0.530 μm) than the PEEK/GL13K (0.634 μm) and PEEK (0.697 μm) samples. The surface of the PEEK/GL13K‐ EDC was more hydrophilic (with a water contact angle of 24°) than the PEEK/GL13K (40°) and pure PEEK (89°) samples. The pure PEEK disc did not exhibit any inhibition zone against S. aureus. After peptide coating, the samples demonstrated significant zones of inhibition: 28 mm and 25 mm for the PEEK/GL13K‐EDC and PEEK/GL13K samples, respectively. The bacteria‐challenged PEEK sample showed numerous bacteria clusters, whereas PEEK/GL13K contained a little bacteria and PEEK/GL13K‐EDC had no bacterial attachment. The results confirm that the GL13K peptide coating was able to induce antibacterial and biofilm‐inhibitory effects. To the best of our knowledge, this is the first report of successful GL13K peptide grafting on a PEEK substrate via EDC coupling. The present work illustrates a facile and promising coating technique for a polymeric surface to provide bactericidal activity and biofilm resistance to medical implantable devices.