Female sex hormones modulate the function of LPS-treated macrophages

Tzu Chieh Chao; Hsiao Hsiang Chao; Miin Fu Chen; John A. Greager; Robert J. Walter

doi:10.1111/j.8755-8920.2000.440511.x

Female sex hormones modulate the function of LPS-treated macrophages

Tzu Chieh Chao^*, Hsiao Hsiang Chao, Miin Fu Chen, John A. Greager, Robert J. Walter

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

41 Scopus citations

Abstract

Problem: To study the effects of estradiol (E₂) or progesterone on macrophage function in the presence of lipopolysaccharide (LPS). Method of study: Male rat peritoneal macrophages were treated in vitro with 0.1 μg/mL of LPS and E₂ or progesterone. Results: At 10^-2 ng/mL, E₂ significantly (P < 0.05; n = 6) enhanced tumor necrosis factor (TNF) release by LPS-treated macrophages. TNF release was significantly (P < 0.05; n = 6) inhibited by 10² ng/mL or 10³ ng/mL of E₂ and by progesterone at less than 10^-3 ng/mL or greater than 10^-1 ng/mL. E₂ (10^-4 and 10 ng/mL) and progesterone (1^-6-10^-4 ng/mL and 10² ng/mL) each significantly (P < 0.05, n = 8) enhanced H₂O₂ release by LPS-treated macrophages. E₂ (< 10^-2 and > 10 ng/mL) and progesterone (10^-7-10⁴ ng/mL) each significantly inhibited (P < 0.05; n = 6) NO₂^- release by LPS-treated macrophages. Conclusions: Exposure to LPS tended to diminish the effects of E₂ and to enhance the effects of progesterone on the parameters determined here. Such LPS-associated alterations in the dose-response profile of macrophages to female sex hormones may contribute to gender-related differences in the immune response under normal and pathological conditions.

Original language	English
Pages (from-to)	310-318
Number of pages	9
Journal	American Journal of Reproductive Immunology
Volume	44
Issue number	5
DOIs	https://doi.org/10.1111/j.8755-8920.2000.440511.x
State	Published - 2000
Externally published	Yes

Keywords

Estrogen
Hydrogen peroxide
Nitric oxide
Progesterone
Tumor necrosis factor

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10.1111/j.8755-8920.2000.440511.x

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title = "Female sex hormones modulate the function of LPS-treated macrophages",

abstract = "Problem: To study the effects of estradiol (E2) or progesterone on macrophage function in the presence of lipopolysaccharide (LPS). Method of study: Male rat peritoneal macrophages were treated in vitro with 0.1 μg/mL of LPS and E2 or progesterone. Results: At 10-2 ng/mL, E2 significantly (P < 0.05; n = 6) enhanced tumor necrosis factor (TNF) release by LPS-treated macrophages. TNF release was significantly (P < 0.05; n = 6) inhibited by 102 ng/mL or 103 ng/mL of E2 and by progesterone at less than 10-3 ng/mL or greater than 10-1 ng/mL. E2 (10-4 and 10 ng/mL) and progesterone (1-6-10-4 ng/mL and 102 ng/mL) each significantly (P < 0.05, n = 8) enhanced H2O2 release by LPS-treated macrophages. E2 (< 10-2 and > 10 ng/mL) and progesterone (10-7-104 ng/mL) each significantly inhibited (P < 0.05; n = 6) NO2- release by LPS-treated macrophages. Conclusions: Exposure to LPS tended to diminish the effects of E2 and to enhance the effects of progesterone on the parameters determined here. Such LPS-associated alterations in the dose-response profile of macrophages to female sex hormones may contribute to gender-related differences in the immune response under normal and pathological conditions.",

keywords = "Estrogen, Hydrogen peroxide, Nitric oxide, Progesterone, Tumor necrosis factor",

author = "Chao, {Tzu Chieh} and Chao, {Hsiao Hsiang} and Chen, {Miin Fu} and Greager, {John A.} and Walter, {Robert J.}",

year = "2000",

doi = "10.1111/j.8755-8920.2000.440511.x",

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journal = "American Journal of Reproductive Immunology",

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T1 - Female sex hormones modulate the function of LPS-treated macrophages

AU - Chao, Tzu Chieh

AU - Chao, Hsiao Hsiang

AU - Chen, Miin Fu

AU - Greager, John A.

AU - Walter, Robert J.

PY - 2000

Y1 - 2000

N2 - Problem: To study the effects of estradiol (E2) or progesterone on macrophage function in the presence of lipopolysaccharide (LPS). Method of study: Male rat peritoneal macrophages were treated in vitro with 0.1 μg/mL of LPS and E2 or progesterone. Results: At 10-2 ng/mL, E2 significantly (P < 0.05; n = 6) enhanced tumor necrosis factor (TNF) release by LPS-treated macrophages. TNF release was significantly (P < 0.05; n = 6) inhibited by 102 ng/mL or 103 ng/mL of E2 and by progesterone at less than 10-3 ng/mL or greater than 10-1 ng/mL. E2 (10-4 and 10 ng/mL) and progesterone (1-6-10-4 ng/mL and 102 ng/mL) each significantly (P < 0.05, n = 8) enhanced H2O2 release by LPS-treated macrophages. E2 (< 10-2 and > 10 ng/mL) and progesterone (10-7-104 ng/mL) each significantly inhibited (P < 0.05; n = 6) NO2- release by LPS-treated macrophages. Conclusions: Exposure to LPS tended to diminish the effects of E2 and to enhance the effects of progesterone on the parameters determined here. Such LPS-associated alterations in the dose-response profile of macrophages to female sex hormones may contribute to gender-related differences in the immune response under normal and pathological conditions.

AB - Problem: To study the effects of estradiol (E2) or progesterone on macrophage function in the presence of lipopolysaccharide (LPS). Method of study: Male rat peritoneal macrophages were treated in vitro with 0.1 μg/mL of LPS and E2 or progesterone. Results: At 10-2 ng/mL, E2 significantly (P < 0.05; n = 6) enhanced tumor necrosis factor (TNF) release by LPS-treated macrophages. TNF release was significantly (P < 0.05; n = 6) inhibited by 102 ng/mL or 103 ng/mL of E2 and by progesterone at less than 10-3 ng/mL or greater than 10-1 ng/mL. E2 (10-4 and 10 ng/mL) and progesterone (1-6-10-4 ng/mL and 102 ng/mL) each significantly (P < 0.05, n = 8) enhanced H2O2 release by LPS-treated macrophages. E2 (< 10-2 and > 10 ng/mL) and progesterone (10-7-104 ng/mL) each significantly inhibited (P < 0.05; n = 6) NO2- release by LPS-treated macrophages. Conclusions: Exposure to LPS tended to diminish the effects of E2 and to enhance the effects of progesterone on the parameters determined here. Such LPS-associated alterations in the dose-response profile of macrophages to female sex hormones may contribute to gender-related differences in the immune response under normal and pathological conditions.

KW - Estrogen

KW - Hydrogen peroxide

KW - Nitric oxide

KW - Progesterone

KW - Tumor necrosis factor

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Female sex hormones modulate the function of LPS-treated macrophages

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