Flexible SERS substrate of silver nanoparticles on cotton swabs for rapid in situ detection of melamine

It is important to be able to detect melamine via a sensitive and fast method in the field of food safety. Surface-enhanced Raman scattering (SERS) has attracted much attention due to its high sensitivity, rapid results, unique spectroscopic fingerprint, and nondestructive data acquisition. In this work, we describe the preparation of flexible CS-ATS-Ag cotton swabs for use in SERS by anchoring silver nanoparticles (AgNPs), as a highly-sensitive SERS material, on cotton swabs (CS) using N-[3-(trimethoxysilyl)propyl] diethylenetriamine (ATS) as the coupling agent. The flexible CS-ATS-Ag cotton swabs exhibited high SERS sensitivity, uniformity and reproducibility as a melamine molecule probe, and the limit of detection was calculated to be 0.2 ppm. A high SERS signal reproducibility was achieved, and the relative standard deviation (RSD) of the melamine peak at 699 cm⁻¹ was approximately 5.01%. Moreover, we successfully developed Chemical analysis App application software; a smartphone was used to convert data and record the results, then the data were geotagged using the GPS feature in the smartphone and uploaded to a central website. The goal of realizing instant transmission, timely processing, high sensitivity, portability and low cost was therefore achieved.