Fluconazole disk diffusion test with methylene blue- and glucoseenriched mueller-hinton agar for determining susceptibility of Candida species

S. C. Lee*, C. P. Fung, N. Lee, L. C. See, J. S. Huang, C. J. Tsai, K. S. Chen, W. B. Shieh

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

31 Scopus citations

Abstract

A 25-μg fluconazole disk diffusion test using a Mueller-Hinton agar plate containing 2% glucose and 5 μg of methylene blue/ml (GM-MH) was compared to the macrodilution reference method for 210 Candida species. The GM-MH agar plate was read at 24 h. The predictive values of disks with susceptible, intermediate, and resistant results on the GM-MH agar plate at 24 h were 97.1, 56.3, and 76.5%, respectively.

Original languageEnglish
Pages (from-to)1615-1617
Number of pages3
JournalJournal of Clinical Microbiology
Volume39
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

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