Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells

Kai Jie Kao, Chien Hsuan Tai, Wen Yi Luo, Ta Sen Yeh, Gwo Bin Lee*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Detection of cancer biomarkers has become increasingly important for the early diagnosis, prognosis, and treatment of cancer. Genetic biomarkers like HER2 have been associated with many types of cancers including breast cancer and gastric cancer. Furthermore, Ilerceptin therapeutics has been found to be particularly effective for patients with an overexpression of HER2 to improve overall survival rates. Fluorescence in situ hybridization (FISH) has been commonly used for HER2 over-expression. However, it requires expensive reagents and skilled personnel. In this work, a novel, integrated FISH microfluidic platform capable of performing the entire FISH protocol automatically to diagnose HER-2 over-expression was reported. Experimental results showed that the developed platform could achieve comparable results when compared with the manual process.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages509-511
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 10 201331 10 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • Cancer biomarker
  • FISH
  • HER2
  • Ilerceptin

Fingerprint

Dive into the research topics of 'Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells'. Together they form a unique fingerprint.

Cite this