Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells

Kai Jie Kao; Chien Hsuan Tai; Wen Yi Luo; Ta Sen Yeh; Gwo Bin Lee

Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells

Kai Jie Kao
, Chien Hsuan Tai
, Wen Yi Luo
, Ta Sen Yeh
, Gwo Bin Lee^*

^*Corresponding author for this work

School of Medicine

National Tsing Hua University
National Cheng Kung University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Detection of cancer biomarkers has become increasingly important for the early diagnosis, prognosis, and treatment of cancer. Genetic biomarkers like HER2 have been associated with many types of cancers including breast cancer and gastric cancer. Furthermore, Ilerceptin therapeutics has been found to be particularly effective for patients with an overexpression of HER2 to improve overall survival rates. Fluorescence in situ hybridization (FISH) has been commonly used for HER2 over-expression. However, it requires expensive reagents and skilled personnel. In this work, a novel, integrated FISH microfluidic platform capable of performing the entire FISH protocol automatically to diagnose HER-2 over-expression was reported. Experimental results showed that the developed platform could achieve comparable results when compared with the manual process.

Original language	English
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Publisher	Chemical and Biological Microsystems Society
Pages	509-511
Number of pages	3
ISBN (Print)	9781632666246
State	Published - 2013
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: 27 10 2013 → 31 10 2013

Publication series

Name	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume	1

Conference

Conference	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/Territory	Germany
City	Freiburg
Period	27/10/13 → 31/10/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer biomarker
FISH
HER2
Ilerceptin

Cite this

Kao, K. J., Tai, C. H., Luo, W. Y., Yeh, T. S., & Lee, G. B. (2013). Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 509-511). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.

Kao, Kai Jie ; Tai, Chien Hsuan ; Luo, Wen Yi et al. / Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 509-511 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).

@inproceedings{3535263123834b9b8baeaddf5cdd8357,

title = "Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells",

abstract = "Detection of cancer biomarkers has become increasingly important for the early diagnosis, prognosis, and treatment of cancer. Genetic biomarkers like HER2 have been associated with many types of cancers including breast cancer and gastric cancer. Furthermore, Ilerceptin therapeutics has been found to be particularly effective for patients with an overexpression of HER2 to improve overall survival rates. Fluorescence in situ hybridization (FISH) has been commonly used for HER2 over-expression. However, it requires expensive reagents and skilled personnel. In this work, a novel, integrated FISH microfluidic platform capable of performing the entire FISH protocol automatically to diagnose HER-2 over-expression was reported. Experimental results showed that the developed platform could achieve comparable results when compared with the manual process.",

keywords = "Cancer biomarker, FISH, HER2, Ilerceptin",

author = "Kao, \{Kai Jie\} and Tai, \{Chien Hsuan\} and Luo, \{Wen Yi\} and Yeh, \{Ta Sen\} and Lee, \{Gwo Bin\}",

year = "2013",

language = "英语",

isbn = "9781632666246",

series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",

publisher = "Chemical and Biological Microsystems Society",

pages = "509--511",

booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",

note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",

}

Kao, KJ, Tai, CH, Luo, WY, Yeh, TS & Lee, GB 2013, Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 1, Chemical and Biological Microsystems Society, pp. 509-511, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 27/10/13.

Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells. / Kao, Kai Jie; Tai, Chien Hsuan; Luo, Wen Yi et al.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 509-511 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells

AU - Kao, Kai Jie

AU - Tai, Chien Hsuan

AU - Luo, Wen Yi

AU - Yeh, Ta Sen

AU - Lee, Gwo Bin

PY - 2013

Y1 - 2013

N2 - Detection of cancer biomarkers has become increasingly important for the early diagnosis, prognosis, and treatment of cancer. Genetic biomarkers like HER2 have been associated with many types of cancers including breast cancer and gastric cancer. Furthermore, Ilerceptin therapeutics has been found to be particularly effective for patients with an overexpression of HER2 to improve overall survival rates. Fluorescence in situ hybridization (FISH) has been commonly used for HER2 over-expression. However, it requires expensive reagents and skilled personnel. In this work, a novel, integrated FISH microfluidic platform capable of performing the entire FISH protocol automatically to diagnose HER-2 over-expression was reported. Experimental results showed that the developed platform could achieve comparable results when compared with the manual process.

AB - Detection of cancer biomarkers has become increasingly important for the early diagnosis, prognosis, and treatment of cancer. Genetic biomarkers like HER2 have been associated with many types of cancers including breast cancer and gastric cancer. Furthermore, Ilerceptin therapeutics has been found to be particularly effective for patients with an overexpression of HER2 to improve overall survival rates. Fluorescence in situ hybridization (FISH) has been commonly used for HER2 over-expression. However, it requires expensive reagents and skilled personnel. In this work, a novel, integrated FISH microfluidic platform capable of performing the entire FISH protocol automatically to diagnose HER-2 over-expression was reported. Experimental results showed that the developed platform could achieve comparable results when compared with the manual process.

KW - Cancer biomarker

KW - FISH

KW - HER2

KW - Ilerceptin

UR - https://www.scopus.com/pages/publications/84907336811

M3 - 会议稿件

AN - SCOPUS:84907336811

SN - 9781632666246

T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

SP - 509

EP - 511

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

PB - Chemical and Biological Microsystems Society

T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Y2 - 27 October 2013 through 31 October 2013

ER -

Kao KJ, Tai CH, Luo WY, Yeh TS, Lee GB. Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 509-511. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).

Fluorescence in situ hybridization (FISH) microfluidic platform for detection of her-2 over-expression in cancer cells

Abstract

Publication series

Conference

UN SDGs

Keywords

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Cite this