TY - JOUR
T1 - Fluoroquinolone Resistance in Clinical Isolates of Streptococcus pneumoniae from Asian Countries
T2 - ANSORP Study
AU - Oh, Won Sup
AU - Suh, Ji Yoeun
AU - Song, Jae Hoon
AU - Ko, Kwan Soo
AU - Jung, Sook In
AU - Peck, Kyong Ran
AU - Lee, Nam Yong
AU - Yang, Yonghong
AU - Chongthaleong, Anan
AU - Chiu, Cheng Hsun
AU - Kamarulzaman, Adeeba
AU - Parasakthi, Navartnam
AU - Lalitha, M. K.
AU - Perera, Jennifer
AU - Yee, Ti Teow
AU - Kumarasinghe, Gamini
AU - Carlos, Celia C.
PY - 2004/3
Y1 - 2004/3
N2 - Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC ≥ 4 μg/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinolone resistance-determining regions (QRDRs) in gyrA, gyrB, parC, and parE. All isolates but one showed more than one amino acid alteration in QRDRs of four responsible genes. Ile460 → Val in parE was the most common mutation. Data suggest that Lys137 → Asn in parC may be a primary step in the development of high-level and multiple FQ resistance. An additional mutation of Ser81 → Phe in gyrA resulted in high-level resistance to ciprofloxacin, levofloxacin, and gatifloxacin, whereas Ser79 → Phe in parC may exert an important role in the development of moxifloxacin resistance. Two novel amino acid changes in gyrB, Ala390 → Val and Asn423 → Thr, were found. Data from PFGE suggest an introduction and local spread of multiple resistant Spain23F-1 clone in Hong Kong, but isolates from other Asian countries were not related to this clone.
AB - Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC ≥ 4 μg/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinolone resistance-determining regions (QRDRs) in gyrA, gyrB, parC, and parE. All isolates but one showed more than one amino acid alteration in QRDRs of four responsible genes. Ile460 → Val in parE was the most common mutation. Data suggest that Lys137 → Asn in parC may be a primary step in the development of high-level and multiple FQ resistance. An additional mutation of Ser81 → Phe in gyrA resulted in high-level resistance to ciprofloxacin, levofloxacin, and gatifloxacin, whereas Ser79 → Phe in parC may exert an important role in the development of moxifloxacin resistance. Two novel amino acid changes in gyrB, Ala390 → Val and Asn423 → Thr, were found. Data from PFGE suggest an introduction and local spread of multiple resistant Spain23F-1 clone in Hong Kong, but isolates from other Asian countries were not related to this clone.
UR - http://www.scopus.com/inward/record.url?scp=11144356592&partnerID=8YFLogxK
U2 - 10.1089/107662904323047781
DO - 10.1089/107662904323047781
M3 - 文章
C2 - 15140392
AN - SCOPUS:11144356592
SN - 1076-6294
VL - 10
SP - 37
EP - 42
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 1
ER -