Fluoxetine-induced Ca2+ signals in Madin-Darby canine kidney cells

K. Y. Tang; J. S. Cheng; K. C. Lee; K. J. Chou; J. K. Huang; W. C. Chen; C. R. Jan

doi:10.1007/s002100000337

Fluoxetine-induced Ca²⁺ signals in Madin-Darby canine kidney cells

K. Y. Tang
, J. S. Cheng
, K. C. Lee
, K. J. Chou
, J. K. Huang
, W. C. Chen
, C. R. Jan^*

^*Corresponding author for this work

Veterans General Hospital-Kaohsiung Taiwan
National Yang Ming Chiao Tung University
Ping Tung Christian Hospital, Taiwan
National Sun Yat-sen University

Research output: Contribution to journal › Journal Article › peer-review

18 Scopus citations

Abstract

The effect of fluoxetine on Ca²⁺ signaling in Madin-Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca²⁺ probe. Fluoxetine increased [Ca²⁺]_i concentration-dependently between 5 μM and 200 μM with an EC₅₀ value of 40 μM. The response was reduced by external Ca²⁺ removal by 30%-40%. In Ca²⁺-free medium pretreatment with 1 μM thapsigargin, an inhibitor of the endoplasmic reticulum Ca²⁺ pump, abolished 100 μM fluoxetine-induced Ca²⁺ release. Addition of 3 mM Ca²⁺ to Ca²⁺-free medium increased [Ca²⁺]_i when cells were pretreated with 100 μM fluoxetine. Suppression of 1,4,5-trisphosphate (IP₃) formation by 2 μM U73122 (a phospholipase C inhibitor) did not affect 100 μM fluoxetine-induced Ca²⁺ release. Fluoxetine (5-100 μM) also increased [Ca²⁺]_i in neutrophils, prostate cancer cells and bladder cancer cells from human and rat glioma cells.

Original language	English
Pages (from-to)	16-20
Number of pages	5
Journal	Naunyn-Schmiedeberg's Archives of Pharmacology
Volume	363
Issue number	1
DOIs	https://doi.org/10.1007/s002100000337
State	Published - 2001
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antidepressant
Ca, intracellular
Fluoxetine
Fura-2
MDCK cells
Serotonim

Access to Document

10.1007/s002100000337

Cite this

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title = "Fluoxetine-induced Ca2+ signals in Madin-Darby canine kidney cells",

abstract = "The effect of fluoxetine on Ca2+ signaling in Madin-Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ probe. Fluoxetine increased [Ca2+]i concentration-dependently between 5 μM and 200 μM with an EC50 value of 40 μM. The response was reduced by external Ca2+ removal by 30\%-40\%. In Ca2+-free medium pretreatment with 1 μM thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+ pump, abolished 100 μM fluoxetine-induced Ca2+ release. Addition of 3 mM Ca2+ to Ca2+-free medium increased [Ca2+]i when cells were pretreated with 100 μM fluoxetine. Suppression of 1,4,5-trisphosphate (IP3) formation by 2 μM U73122 (a phospholipase C inhibitor) did not affect 100 μM fluoxetine-induced Ca2+ release. Fluoxetine (5-100 μM) also increased [Ca2+]i in neutrophils, prostate cancer cells and bladder cancer cells from human and rat glioma cells.",

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author = "Tang, \{K. Y.\} and Cheng, \{J. S.\} and Lee, \{K. C.\} and Chou, \{K. J.\} and Huang, \{J. K.\} and Chen, \{W. C.\} and Jan, \{C. R.\}",

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T1 - Fluoxetine-induced Ca2+ signals in Madin-Darby canine kidney cells

AU - Tang, K. Y.

AU - Cheng, J. S.

AU - Lee, K. C.

AU - Chou, K. J.

AU - Huang, J. K.

AU - Chen, W. C.

AU - Jan, C. R.

PY - 2001

Y1 - 2001

N2 - The effect of fluoxetine on Ca2+ signaling in Madin-Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ probe. Fluoxetine increased [Ca2+]i concentration-dependently between 5 μM and 200 μM with an EC50 value of 40 μM. The response was reduced by external Ca2+ removal by 30%-40%. In Ca2+-free medium pretreatment with 1 μM thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+ pump, abolished 100 μM fluoxetine-induced Ca2+ release. Addition of 3 mM Ca2+ to Ca2+-free medium increased [Ca2+]i when cells were pretreated with 100 μM fluoxetine. Suppression of 1,4,5-trisphosphate (IP3) formation by 2 μM U73122 (a phospholipase C inhibitor) did not affect 100 μM fluoxetine-induced Ca2+ release. Fluoxetine (5-100 μM) also increased [Ca2+]i in neutrophils, prostate cancer cells and bladder cancer cells from human and rat glioma cells.

AB - The effect of fluoxetine on Ca2+ signaling in Madin-Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ probe. Fluoxetine increased [Ca2+]i concentration-dependently between 5 μM and 200 μM with an EC50 value of 40 μM. The response was reduced by external Ca2+ removal by 30%-40%. In Ca2+-free medium pretreatment with 1 μM thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+ pump, abolished 100 μM fluoxetine-induced Ca2+ release. Addition of 3 mM Ca2+ to Ca2+-free medium increased [Ca2+]i when cells were pretreated with 100 μM fluoxetine. Suppression of 1,4,5-trisphosphate (IP3) formation by 2 μM U73122 (a phospholipase C inhibitor) did not affect 100 μM fluoxetine-induced Ca2+ release. Fluoxetine (5-100 μM) also increased [Ca2+]i in neutrophils, prostate cancer cells and bladder cancer cells from human and rat glioma cells.

KW - Antidepressant

KW - Ca, intracellular

KW - Fluoxetine

KW - Fura-2

KW - MDCK cells

KW - Serotonim

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DO - 10.1007/s002100000337

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