Focused shockwave induced blood-brain barrier opening and transfection

Yi Kung; Chiang Lan; Ming Yen Hsiao; Ming Kuan Sun; Yi Hua Hsu; Abel P.H. Huang; Wei Hao Liao; Hao Li Liu; Claude Inserra; Wen Shiang Chen

doi:10.1038/s41598-018-20672-y

Focused shockwave induced blood-brain barrier opening and transfection

Yi Kung
, Chiang Lan
, Ming Yen Hsiao
, Ming Kuan Sun
, Yi Hua Hsu
, Abel P.H. Huang
, Wei Hao Liao
, Hao Li Liu
, Claude Inserra
, Wen Shiang Chen^*

^*Corresponding author for this work

Department of Electrical Engineering

Research output: Contribution to journal › Journal Article › peer-review

30 Scopus citations

Abstract

Despite extensive efforts in recent years, the blood-brain barrier (BBB) remains a significant obstacle for drug delivery. This study proposes using a clinical extracorporeal shockwave instrument to open the BBB, combined with a laser assisted bi-axial locating platform to achieve non-invasive, controllable-focus and reversible BBB opening in the brains of rats. Under shockwave treatment with an intensity level of 5 (P^-9.79 MPa, energy flux density (EFD) 0.21 mJ/mm²) and a pulse repetition frequency of 5 Hz, the BBB could be opened after 50 shocks without the use of an ultrasound contrast agent. With the proposed method, the BBB opening can be precisely controlled in terms of depth, size and location. Moreover, a shockwave based gene transfection was demonstrated using a luciferase gene.

Original language	English
Article number	2218
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-20672-y
State	Published - 01 12 2018

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© 2018 The Author(s).

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title = "Focused shockwave induced blood-brain barrier opening and transfection",

abstract = "Despite extensive efforts in recent years, the blood-brain barrier (BBB) remains a significant obstacle for drug delivery. This study proposes using a clinical extracorporeal shockwave instrument to open the BBB, combined with a laser assisted bi-axial locating platform to achieve non-invasive, controllable-focus and reversible BBB opening in the brains of rats. Under shockwave treatment with an intensity level of 5 (P-9.79 MPa, energy flux density (EFD) 0.21 mJ/mm2) and a pulse repetition frequency of 5 Hz, the BBB could be opened after 50 shocks without the use of an ultrasound contrast agent. With the proposed method, the BBB opening can be precisely controlled in terms of depth, size and location. Moreover, a shockwave based gene transfection was demonstrated using a luciferase gene.",

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doi = "10.1038/s41598-018-20672-y",

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AU - Kung, Yi

AU - Lan, Chiang

AU - Hsiao, Ming Yen

AU - Sun, Ming Kuan

AU - Hsu, Yi Hua

AU - Huang, Abel P.H.

AU - Liao, Wei Hao

AU - Liu, Hao Li

AU - Inserra, Claude

AU - Chen, Wen Shiang

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Despite extensive efforts in recent years, the blood-brain barrier (BBB) remains a significant obstacle for drug delivery. This study proposes using a clinical extracorporeal shockwave instrument to open the BBB, combined with a laser assisted bi-axial locating platform to achieve non-invasive, controllable-focus and reversible BBB opening in the brains of rats. Under shockwave treatment with an intensity level of 5 (P-9.79 MPa, energy flux density (EFD) 0.21 mJ/mm2) and a pulse repetition frequency of 5 Hz, the BBB could be opened after 50 shocks without the use of an ultrasound contrast agent. With the proposed method, the BBB opening can be precisely controlled in terms of depth, size and location. Moreover, a shockwave based gene transfection was demonstrated using a luciferase gene.

AB - Despite extensive efforts in recent years, the blood-brain barrier (BBB) remains a significant obstacle for drug delivery. This study proposes using a clinical extracorporeal shockwave instrument to open the BBB, combined with a laser assisted bi-axial locating platform to achieve non-invasive, controllable-focus and reversible BBB opening in the brains of rats. Under shockwave treatment with an intensity level of 5 (P-9.79 MPa, energy flux density (EFD) 0.21 mJ/mm2) and a pulse repetition frequency of 5 Hz, the BBB could be opened after 50 shocks without the use of an ultrasound contrast agent. With the proposed method, the BBB opening can be precisely controlled in terms of depth, size and location. Moreover, a shockwave based gene transfection was demonstrated using a luciferase gene.

UR - https://www.scopus.com/pages/publications/85041558908

U2 - 10.1038/s41598-018-20672-y

DO - 10.1038/s41598-018-20672-y

M3 - 文章

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AN - SCOPUS:85041558908

SN - 2045-2322

VL - 8

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 2218

ER -

Focused shockwave induced blood-brain barrier opening and transfection

Abstract

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