Functionalized temperature-sensitive copolymer for tissue engineering of articular cartilage and meniscus

Jyh Ping Chen; Tai Hong Cheng

doi:10.1016/j.colsurfa.2007.04.130

Functionalized temperature-sensitive copolymer for tissue engineering of articular cartilage and meniscus

Jyh Ping Chen^*, Tai Hong Cheng

^*Corresponding author for this work

Department of Chemical and Materials Engineering

Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

23 Scopus citations

Abstract

Thermo-sensitive chitosan-g-poly(N-isopropylacrylamide) (CPN) and hyaluronic acid-g-CPN (HA-CPN) copolymer were fabricated by forming amide bond linkages between carboxyl-ended poly(N-isopropylacrylamide), chitosan, and HA. Aqueous polymer solutions showed reversibly soluble-insoluble phase transition characteristics, which is free-flowing at room temperature and turns into a rigid gel structure at 37 °C. SEM observation demonstrated an inter-connected microporous structure with pore-size ranging from 20 to 50 μm. Chondrocytes and meniscus cells attached and proliferated well when cultured on the copolymer hydrogel. The cells maintained high viability and showed distinct morphology. The aggregated chondrocytes on the thermo-reversible hydrogel surface can be easily detached and recovered by simply lowering the temperature.

Original language	English
Pages (from-to)	254-259
Number of pages	6
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	313-314
DOIs	https://doi.org/10.1016/j.colsurfa.2007.04.130
State	Published - 01 02 2008

Keywords

Hydrogel
N-Isopropylacrylamide
Temperature-sensitive copolymer
Tissue engineering

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10.1016/j.colsurfa.2007.04.130

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title = "Functionalized temperature-sensitive copolymer for tissue engineering of articular cartilage and meniscus",

abstract = "Thermo-sensitive chitosan-g-poly(N-isopropylacrylamide) (CPN) and hyaluronic acid-g-CPN (HA-CPN) copolymer were fabricated by forming amide bond linkages between carboxyl-ended poly(N-isopropylacrylamide), chitosan, and HA. Aqueous polymer solutions showed reversibly soluble-insoluble phase transition characteristics, which is free-flowing at room temperature and turns into a rigid gel structure at 37 °C. SEM observation demonstrated an inter-connected microporous structure with pore-size ranging from 20 to 50 μm. Chondrocytes and meniscus cells attached and proliferated well when cultured on the copolymer hydrogel. The cells maintained high viability and showed distinct morphology. The aggregated chondrocytes on the thermo-reversible hydrogel surface can be easily detached and recovered by simply lowering the temperature.",

keywords = "Hydrogel, N-Isopropylacrylamide, Temperature-sensitive copolymer, Tissue engineering",

author = "Chen, {Jyh Ping} and Cheng, {Tai Hong}",

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doi = "10.1016/j.colsurfa.2007.04.130",

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T1 - Functionalized temperature-sensitive copolymer for tissue engineering of articular cartilage and meniscus

AU - Chen, Jyh Ping

AU - Cheng, Tai Hong

PY - 2008/2/1

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N2 - Thermo-sensitive chitosan-g-poly(N-isopropylacrylamide) (CPN) and hyaluronic acid-g-CPN (HA-CPN) copolymer were fabricated by forming amide bond linkages between carboxyl-ended poly(N-isopropylacrylamide), chitosan, and HA. Aqueous polymer solutions showed reversibly soluble-insoluble phase transition characteristics, which is free-flowing at room temperature and turns into a rigid gel structure at 37 °C. SEM observation demonstrated an inter-connected microporous structure with pore-size ranging from 20 to 50 μm. Chondrocytes and meniscus cells attached and proliferated well when cultured on the copolymer hydrogel. The cells maintained high viability and showed distinct morphology. The aggregated chondrocytes on the thermo-reversible hydrogel surface can be easily detached and recovered by simply lowering the temperature.

AB - Thermo-sensitive chitosan-g-poly(N-isopropylacrylamide) (CPN) and hyaluronic acid-g-CPN (HA-CPN) copolymer were fabricated by forming amide bond linkages between carboxyl-ended poly(N-isopropylacrylamide), chitosan, and HA. Aqueous polymer solutions showed reversibly soluble-insoluble phase transition characteristics, which is free-flowing at room temperature and turns into a rigid gel structure at 37 °C. SEM observation demonstrated an inter-connected microporous structure with pore-size ranging from 20 to 50 μm. Chondrocytes and meniscus cells attached and proliferated well when cultured on the copolymer hydrogel. The cells maintained high viability and showed distinct morphology. The aggregated chondrocytes on the thermo-reversible hydrogel surface can be easily detached and recovered by simply lowering the temperature.

KW - Hydrogel

KW - N-Isopropylacrylamide

KW - Temperature-sensitive copolymer

KW - Tissue engineering

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U2 - 10.1016/j.colsurfa.2007.04.130

DO - 10.1016/j.colsurfa.2007.04.130

M3 - 文章

AN - SCOPUS:37349057058

SN - 0927-7757

VL - 313-314

SP - 254

EP - 259

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

ER -

Functionalized temperature-sensitive copolymer for tissue engineering of articular cartilage and meniscus

Abstract

Keywords

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