GATA-1 and NF-Y cooperate to mediate erythroid-specific transcription of Gfi-1B gene

Duen Yi Huang, Yuan Yeh Kuo, Jiann Shiun Lai, Yutaka Suzuki, Sumio Sugano, Zee Fen Chang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

41 Scopus citations

Abstract

Expression of Gfi (growth factor-independence)-1B, a Gfi-1-related transcriptional repressor, is restricted to erythroid lineage cells and is essential for erythropoiesis. We have determined the transcription start site of the human Gfi-1B gene and located its first non-coding exon ∼7.82 kb upstream of the first coding exon. The genomic sequence preceding this first non-coding exon has been identified to be its erythroid-specific promoter region in K562 cells. Using gel-shift and chromatin immunoprecipitation (ChlP) assays, we have demonstrated that NF-Y and GATA-1 directly participate in transcriptional activation of the Gfi-1B gene in K562 cells. Ectopic expression of GATA-1 markedly stimulates the activity of the Gfi-1B promoter in a non-erythroid cell line U937. Interestingly, our results have indicated that this GATA-1-mediated transactivation is dependent on NF-Y binding to the CCAAT site. Here we conclude that functional cooperation between GATA-1 and NF-Y contributes to erythroid-specific transcriptional activation of Gfi-1B promoter.

Original languageEnglish
Pages (from-to)3935-3946
Number of pages12
JournalNucleic Acids Research
Volume32
Issue number13
DOIs
StatePublished - 2004
Externally publishedYes

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