TY - JOUR
T1 - Genetic programs expressed in resting and IL-4 alternatively activated mouse and human macrophages
T2 - Similarities and differences
AU - Martinez, Fernando O.
AU - Helming, Laura
AU - Milde, Ronny
AU - Varin, Audrey
AU - Melgert, Barbro N.
AU - Draijer, Christina
AU - Thomas, Benjamin
AU - Fabbri, Marco
AU - Crawshaw, Anjali
AU - Ho, Ling Pei
AU - Hacken, Nick H.Ten
AU - Jiménez, Viviana Cobos
AU - Kootstra, Neeltje A.
AU - Hamann, Jörg
AU - Greaves, David R.
AU - Locati, Massimo
AU - Mantovani, Alberto
AU - Gordon, Siamon
PY - 2013/2/28
Y1 - 2013/2/28
N2 - The molecular repertoire of macrophages in health and disease can provide novel biomarkers for diagnosis, prognosis, and treatment. Th2-IL-4-activated macrophages (M2) have been associated with important diseases in mice, yet no specific markers are available for their detection in human tissues. Although mouse models are widely used for macrophage research, translation to the human can be problematic and the human macrophage system remains poorly described. In the present study, we analyzed and compared the transcriptome and proteome ofhumanand murine macrophages under resting conditions (M0) and after IL-4 activation (M2). We provide a resource for tools enabling macrophage detection in human tissues by identifying a set of 87 macrophage-related genes. Furthermore, we extend current understanding of M2 activation in different species and identify Transglutaminase 2 as a conserved M2 marker that is highly expressed by human macrophages and monocytes in the prototypic Th2 pathology asthma.
AB - The molecular repertoire of macrophages in health and disease can provide novel biomarkers for diagnosis, prognosis, and treatment. Th2-IL-4-activated macrophages (M2) have been associated with important diseases in mice, yet no specific markers are available for their detection in human tissues. Although mouse models are widely used for macrophage research, translation to the human can be problematic and the human macrophage system remains poorly described. In the present study, we analyzed and compared the transcriptome and proteome ofhumanand murine macrophages under resting conditions (M0) and after IL-4 activation (M2). We provide a resource for tools enabling macrophage detection in human tissues by identifying a set of 87 macrophage-related genes. Furthermore, we extend current understanding of M2 activation in different species and identify Transglutaminase 2 as a conserved M2 marker that is highly expressed by human macrophages and monocytes in the prototypic Th2 pathology asthma.
UR - http://www.scopus.com/inward/record.url?scp=84875658660&partnerID=8YFLogxK
U2 - 10.1182/blood-2012-06-436212
DO - 10.1182/blood-2012-06-436212
M3 - 文章
C2 - 23293084
AN - SCOPUS:84875658660
SN - 0006-4971
VL - 121
SP - e57-e69
JO - Blood
JF - Blood
IS - 9
ER -
