Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor

K. Kaushansky; P. J. O'Hara; K. Berkner; G. M. Segal; F. S. Hagen; J. W. Adamson

doi:10.1073/pnas.83.10.3101

Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor

K. Kaushansky
, P. J. O'Hara
, K. Berkner
, G. M. Segal
, F. S. Hagen
, J. W. Adamson

University of Washington

Research output: Contribution to journal › Journal Article › peer-review

143 Scopus citations

Abstract

Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.

Original language	English
Pages (from-to)	3101-3105
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	83
Issue number	10
DOIs	https://doi.org/10.1073/pnas.83.10.3101
State	Published - 1986
Externally published	Yes

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Kaushansky, K., O'Hara, P. J., Berkner, K., Segal, G. M., Hagen, F. S., & Adamson, J. W. (1986). Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor. Proceedings of the National Academy of Sciences of the United States of America, 83(10), 3101-3105. https://doi.org/10.1073/pnas.83.10.3101

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title = "Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor",

abstract = "Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.",

author = "K. Kaushansky and O'Hara, \{P. J.\} and K. Berkner and Segal, \{G. M.\} and Hagen, \{F. S.\} and Adamson, \{J. W.\}",

year = "1986",

doi = "10.1073/pnas.83.10.3101",

language = "英语",

volume = "83",

pages = "3101--3105",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "10",

}

Kaushansky, K, O'Hara, PJ, Berkner, K, Segal, GM, Hagen, FS & Adamson, JW 1986, 'Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor', Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 10, pp. 3101-3105. https://doi.org/10.1073/pnas.83.10.3101

Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor. / Kaushansky, K.; O'Hara, P. J.; Berkner, K. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 10, 1986, p. 3101-3105.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor

AU - Kaushansky, K.

AU - O'Hara, P. J.

AU - Berkner, K.

AU - Segal, G. M.

AU - Hagen, F. S.

AU - Adamson, J. W.

PY - 1986

Y1 - 1986

N2 - Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.

AB - Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.

UR - https://www.scopus.com/pages/publications/0022495791

U2 - 10.1073/pnas.83.10.3101

DO - 10.1073/pnas.83.10.3101

M3 - 文章

AN - SCOPUS:0022495791

SN - 0027-8424

VL - 83

SP - 3101

EP - 3105

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 10

ER -

Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor

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