Genomic organization and characterization of the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) JAK1 kinase gene

Jiann Horng Leu; Mau Sun Chang; Chen Wen Yao; Chen Kung Chou; Shui Tsung Chen; Chang Jen Huang

doi:10.1016/S0167-4781(97)00137-1

Genomic organization and characterization of the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) JAK1 kinase gene

Jiann Horng Leu, Mau Sun Chang, Chen Wen Yao, Chen Kung Chou, Shui Tsung Chen, Chang Jen Huang^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

23 Scopus citations

Abstract

Seventeen kilobases of genomic DNA containing the promoter and the coding region of the round-spotted pufferfish JAK1 gene was isolated and completely sequenced. This gene consists of 25 exons and 24 introns spanning about 13.5 kb, compared to > 30kb in carp JAK1 gene. Primer extension analysis revealed one transcription initiation site which was 376 bp upstream of the translation initiation site. The sequence of the 2.9 kb region upstream of the transcription initiation site contains numerous potential binding sites for transcription factors including HNF-5, GCF, Sp1, CRE, AP2, GATA, GAGA, E2A, p53, and NF-IL6. When this region was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF call line, it could drive the synthesis of CAT enzyme three times more efficiently than could the common carp JAK1 promoter.

Original language	English
Pages (from-to)	50-56
Number of pages	7
Journal	BBA - Gene Structure and Expression
Volume	1395
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(97)00137-1
State	Published - 07 01 1998
Externally published	Yes

Keywords

(Round-spotted pufferfish)
(Tetraodon fluviatilis)
Genomic structure
JAK1 kinase
Promoter

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10.1016/S0167-4781(97)00137-1

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title = "Genomic organization and characterization of the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) JAK1 kinase gene",

abstract = "Seventeen kilobases of genomic DNA containing the promoter and the coding region of the round-spotted pufferfish JAK1 gene was isolated and completely sequenced. This gene consists of 25 exons and 24 introns spanning about 13.5 kb, compared to > 30kb in carp JAK1 gene. Primer extension analysis revealed one transcription initiation site which was 376 bp upstream of the translation initiation site. The sequence of the 2.9 kb region upstream of the transcription initiation site contains numerous potential binding sites for transcription factors including HNF-5, GCF, Sp1, CRE, AP2, GATA, GAGA, E2A, p53, and NF-IL6. When this region was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF call line, it could drive the synthesis of CAT enzyme three times more efficiently than could the common carp JAK1 promoter.",

keywords = "(Round-spotted pufferfish), (Tetraodon fluviatilis), Genomic structure, JAK1 kinase, Promoter",

author = "Leu, \{Jiann Horng\} and Chang, \{Mau Sun\} and Yao, \{Chen Wen\} and Chou, \{Chen Kung\} and Chen, \{Shui Tsung\} and Huang, \{Chang Jen\}",

year = "1998",

month = jan,

day = "7",

doi = "10.1016/S0167-4781(97)00137-1",

language = "英语",

volume = "1395",

pages = "50--56",

journal = "BBA - Gene Structure and Expression",

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TY - JOUR

T1 - Genomic organization and characterization of the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) JAK1 kinase gene

AU - Leu, Jiann Horng

AU - Chang, Mau Sun

AU - Yao, Chen Wen

AU - Chou, Chen Kung

AU - Chen, Shui Tsung

AU - Huang, Chang Jen

PY - 1998/1/7

Y1 - 1998/1/7

N2 - Seventeen kilobases of genomic DNA containing the promoter and the coding region of the round-spotted pufferfish JAK1 gene was isolated and completely sequenced. This gene consists of 25 exons and 24 introns spanning about 13.5 kb, compared to > 30kb in carp JAK1 gene. Primer extension analysis revealed one transcription initiation site which was 376 bp upstream of the translation initiation site. The sequence of the 2.9 kb region upstream of the transcription initiation site contains numerous potential binding sites for transcription factors including HNF-5, GCF, Sp1, CRE, AP2, GATA, GAGA, E2A, p53, and NF-IL6. When this region was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF call line, it could drive the synthesis of CAT enzyme three times more efficiently than could the common carp JAK1 promoter.

AB - Seventeen kilobases of genomic DNA containing the promoter and the coding region of the round-spotted pufferfish JAK1 gene was isolated and completely sequenced. This gene consists of 25 exons and 24 introns spanning about 13.5 kb, compared to > 30kb in carp JAK1 gene. Primer extension analysis revealed one transcription initiation site which was 376 bp upstream of the translation initiation site. The sequence of the 2.9 kb region upstream of the transcription initiation site contains numerous potential binding sites for transcription factors including HNF-5, GCF, Sp1, CRE, AP2, GATA, GAGA, E2A, p53, and NF-IL6. When this region was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF call line, it could drive the synthesis of CAT enzyme three times more efficiently than could the common carp JAK1 promoter.

KW - (Round-spotted pufferfish)

KW - (Tetraodon fluviatilis)

KW - Genomic structure

KW - JAK1 kinase

KW - Promoter

UR - https://www.scopus.com/pages/publications/0032491908

U2 - 10.1016/S0167-4781(97)00137-1

DO - 10.1016/S0167-4781(97)00137-1

M3 - 文章

C2 - 9434151

AN - SCOPUS:0032491908

SN - 0167-4781

VL - 1395

SP - 50

EP - 56

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 1

ER -

Genomic organization and characterization of the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) JAK1 kinase gene

Abstract

Keywords

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