Global Genome Diversity and Recombination in Mycoplasma pneumoniae

Yu Chia Hsieh; Shiao Wen Li; Yi Yin Chen; Ching Chia Kuo; Yin Cheng Chen; Ian Yi Feng Chang; Yi Jiun Pan; Ting Hsuan Li; Ruei Lin Chiang; Ya Yu Huang; Wei Chao Liao

doi:10.3201/eid2801.210497

Global Genome Diversity and Recombination in Mycoplasma pneumoniae

Yu Chia Hsieh, Shiao Wen Li, Yi Yin Chen, Ching Chia Kuo, Yin Cheng Chen, Ian Yi Feng Chang, Yi Jiun Pan, Ting Hsuan Li, Ruei Lin Chiang, Ya Yu Huang, Wei Chao Liao^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

21 Scopus citations

Abstract

Genomic changes in Mycoplasma pneumoniae caused by adaptation to environmental or ecologic pressures are poorly understood. We collected M. pneumoniae from children who had confi rmed pneumonia in Taiwan during 2017-2020. We used whole-genome sequencing to compare these isolates with a worldwide collection of current and historical clinical strains for characterizing population structures. A phylogenetic tree for 284 strains showed that all sequenced strains consisted of 5 clades: T1-1 (sequence type [ST]1), T1-2 (mainly ST3), T1-3 (ST17), T2-1 (mainly ST2), and T2-2 (mainly ST14). We identifi ed a putative recombination block containing 6 genes (MPN366-371). Macrolide resistance involving 23S rRNA mutations was detected for each clade. Clonal expansion of macrolide resistance occurred mostly within subtype 1 strains, of which clade T1-2 showed the highest recombination rate and genome diversity. Functional characterization of recombined regions provided clarifi cation of the biologic role of these recombination events in the evolution of M. pneumoniae.

Original language	English
Pages (from-to)	111-117
Number of pages	7
Journal	Emerging Infectious Diseases
Volume	28
Issue number	1
DOIs	https://doi.org/10.3201/eid2801.210497
State	Published - 01 2022

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© 2022 Centers for Disease Control and Prevention (CDC). All rights reserved.

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title = "Global Genome Diversity and Recombination in Mycoplasma pneumoniae",

abstract = "Genomic changes in Mycoplasma pneumoniae caused by adaptation to environmental or ecologic pressures are poorly understood. We collected M. pneumoniae from children who had confi rmed pneumonia in Taiwan during 2017-2020. We used whole-genome sequencing to compare these isolates with a worldwide collection of current and historical clinical strains for characterizing population structures. A phylogenetic tree for 284 strains showed that all sequenced strains consisted of 5 clades: T1-1 (sequence type [ST]1), T1-2 (mainly ST3), T1-3 (ST17), T2-1 (mainly ST2), and T2-2 (mainly ST14). We identifi ed a putative recombination block containing 6 genes (MPN366-371). Macrolide resistance involving 23S rRNA mutations was detected for each clade. Clonal expansion of macrolide resistance occurred mostly within subtype 1 strains, of which clade T1-2 showed the highest recombination rate and genome diversity. Functional characterization of recombined regions provided clarifi cation of the biologic role of these recombination events in the evolution of M. pneumoniae.",

author = "Hsieh, \{Yu Chia\} and Li, \{Shiao Wen\} and Chen, \{Yi Yin\} and Kuo, \{Ching Chia\} and Chen, \{Yin Cheng\} and Chang, \{Ian Yi Feng\} and Pan, \{Yi Jiun\} and Li, \{Ting Hsuan\} and Chiang, \{Ruei Lin\} and Huang, \{Ya Yu\} and Liao, \{Wei Chao\}",

year = "2022",

month = jan,

doi = "10.3201/eid2801.210497",

language = "英语",

volume = "28",

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T1 - Global Genome Diversity and Recombination in Mycoplasma pneumoniae

AU - Hsieh, Yu Chia

AU - Li, Shiao Wen

AU - Chen, Yi Yin

AU - Kuo, Ching Chia

AU - Chen, Yin Cheng

AU - Chang, Ian Yi Feng

AU - Pan, Yi Jiun

AU - Li, Ting Hsuan

AU - Chiang, Ruei Lin

AU - Huang, Ya Yu

AU - Liao, Wei Chao

PY - 2022/1

Y1 - 2022/1

N2 - Genomic changes in Mycoplasma pneumoniae caused by adaptation to environmental or ecologic pressures are poorly understood. We collected M. pneumoniae from children who had confi rmed pneumonia in Taiwan during 2017-2020. We used whole-genome sequencing to compare these isolates with a worldwide collection of current and historical clinical strains for characterizing population structures. A phylogenetic tree for 284 strains showed that all sequenced strains consisted of 5 clades: T1-1 (sequence type [ST]1), T1-2 (mainly ST3), T1-3 (ST17), T2-1 (mainly ST2), and T2-2 (mainly ST14). We identifi ed a putative recombination block containing 6 genes (MPN366-371). Macrolide resistance involving 23S rRNA mutations was detected for each clade. Clonal expansion of macrolide resistance occurred mostly within subtype 1 strains, of which clade T1-2 showed the highest recombination rate and genome diversity. Functional characterization of recombined regions provided clarifi cation of the biologic role of these recombination events in the evolution of M. pneumoniae.

AB - Genomic changes in Mycoplasma pneumoniae caused by adaptation to environmental or ecologic pressures are poorly understood. We collected M. pneumoniae from children who had confi rmed pneumonia in Taiwan during 2017-2020. We used whole-genome sequencing to compare these isolates with a worldwide collection of current and historical clinical strains for characterizing population structures. A phylogenetic tree for 284 strains showed that all sequenced strains consisted of 5 clades: T1-1 (sequence type [ST]1), T1-2 (mainly ST3), T1-3 (ST17), T2-1 (mainly ST2), and T2-2 (mainly ST14). We identifi ed a putative recombination block containing 6 genes (MPN366-371). Macrolide resistance involving 23S rRNA mutations was detected for each clade. Clonal expansion of macrolide resistance occurred mostly within subtype 1 strains, of which clade T1-2 showed the highest recombination rate and genome diversity. Functional characterization of recombined regions provided clarifi cation of the biologic role of these recombination events in the evolution of M. pneumoniae.

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DO - 10.3201/eid2801.210497

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SN - 1080-6040

VL - 28

SP - 111

EP - 117

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 1

ER -

Global Genome Diversity and Recombination in Mycoplasma pneumoniae

Abstract

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