Glycated type 1 collagen induces endothelial dysfunction in culture

Pei Ching Kuo, Cheng Heng Kao, Jan Kan Chen*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

4 Scopus citations


Hyperglycemia-induced protein glycation is thought to be implicated in the diabetic vasculopathy. In this study, we cultured vascular endothelial cells on native or glycated collagen matrix and compared their growth and functional characteristics. At lower plating density, the cells grew equally well on both substrata; however, at higher planting density, the cells plated on glycated collagen grew slower and reached a lower confluent density compared to that of the native collagen-based cultures. Confluent cell layers formed on glycated collagen exhibited a lower diffusion barrier function and a less response to epidermal growth factor stimulated prostacyclin production, compared to their native collagen-cultured counterparts.

Original languageEnglish
Pages (from-to)338-343
Number of pages6
JournalIn Vitro Cellular & Developmental Biology - Animal
Issue number10
StatePublished - 12 2007


  • EGF
  • Hyperglycemia
  • Prostacyclin


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