Gold nanoparticle-based redox signal enhancement towards the detection of single bacterium

Jen Kuei Wu, Hwan You Chang, Fan Gang Tseng*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this work, we report a portable microfluidic chip based on gold nanoparticle-enhanced electrochemical detection for single-bacteria diognostic. The possibility of single-bacteria detection is realized here by using electroactive molecules modified on gold nanoparticles (NPs) to detect and differentiate Staphylococcus aureus and Pseudomonas aeruginosa. The detection limit of these two electroactive molecules is near femtomolar (1 pM) when immobilized on gold nanoparticles (NPs), and the minima amounts of gold NPs required in the detection is around thousands (103 particle/l). A concentration of couples whole bacteria (<10 cells/l) is detected by CV measurements. The liner detection limit concention is form 1-1000 cells/μl. The results showed that the portable biochip system has great potential as a device for single-particle or possibly even single-organism detection.

Original languageEnglish
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages2758-2761
Number of pages4
DOIs
StatePublished - 2011
Externally publishedYes
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: 05 06 201109 06 2011

Publication series

Name2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Conference

Conference2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Country/TerritoryChina
CityBeijing
Period05/06/1109/06/11

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