Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip

Yin Yun Chen; Jyun Wei Chen; Jie Sheng Chen; Yi Ching Ko; Hsiang Hao Hsu; Fan Gang Tseng

Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip

Yin Yun Chen
, Jyun Wei Chen
, Jie Sheng Chen
, Yi Ching Ko
, Hsiang Hao Hsu
, Fan Gang Tseng^*

^*Corresponding author for this work

School of Medicine

National Tsing Hua University
Chang Gung Memorial Hospital
Academia Sinica - Research Center for Applied Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In this research, we demonstrate successful glomerular cells cultivation outside co-shelled alginate tubes rather than cells cultivation inside the alginate tubes to reduce the interference of growth space on cell proliferation and differentiation. Podocytes were seeded on the outerior of an alginate tube by a tube infiltration method into a cell culturing site, and cells were successfully attached on the outer of the alginate tube and formed tubular structures instead of a cellular microfiber tube after 7 days cultivation. The reconstructed glomerular tube will be used as an in-vitro model for drug testing, and also the first step toward re-establishing a fully functional artificial glomerular.

Original language	English
Title of host publication	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Publisher	Chemical and Biological Microsystems Society
Pages	1596-1597
Number of pages	2
ISBN (Electronic)	9781733419000
State	Published - 2019
Event	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland Duration: 27 10 2019 → 31 10 2019

Publication series

Name	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/Territory	Switzerland
City	Basel
Period	27/10/19 → 31/10/19

Bibliographical note

Publisher Copyright:
© 2019 CBMS-0001.

Keywords

Alginate
Co-shelled tube
Podocyte
Tissue Engineering

Cite this

Chen, Y. Y., Chen, J. W., Chen, J. S., Ko, Y. C., Hsu, H. H., & Tseng, F. G. (2019). Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 1596-1597). (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019). Chemical and Biological Microsystems Society.

Chen, Yin Yun ; Chen, Jyun Wei ; Chen, Jie Sheng et al. / Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. pp. 1596-1597 (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

@inproceedings{e47da0319c814c11b3f153152203f23d,

title = "Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip",

abstract = "In this research, we demonstrate successful glomerular cells cultivation outside co-shelled alginate tubes rather than cells cultivation inside the alginate tubes to reduce the interference of growth space on cell proliferation and differentiation. Podocytes were seeded on the outerior of an alginate tube by a tube infiltration method into a cell culturing site, and cells were successfully attached on the outer of the alginate tube and formed tubular structures instead of a cellular microfiber tube after 7 days cultivation. The reconstructed glomerular tube will be used as an in-vitro model for drug testing, and also the first step toward re-establishing a fully functional artificial glomerular.",

keywords = "Alginate, Co-shelled tube, Podocyte, Tissue Engineering",

author = "Chen, \{Yin Yun\} and Chen, \{Jyun Wei\} and Chen, \{Jie Sheng\} and Ko, \{Yi Ching\} and Hsu, \{Hsiang Hao\} and Tseng, \{Fan Gang\}",

note = "Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

language = "英语",

series = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

publisher = "Chemical and Biological Microsystems Society",

pages = "1596--1597",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

}

Chen, YY, Chen, JW, Chen, JS, Ko, YC, Hsu, HH & Tseng, FG 2019, Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Chemical and Biological Microsystems Society, pp. 1596-1597, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Basel, Switzerland, 27/10/19.

Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip. / Chen, Yin Yun; Chen, Jyun Wei; Chen, Jie Sheng et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. p. 1596-1597 (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip

AU - Chen, Yin Yun

AU - Chen, Jyun Wei

AU - Chen, Jie Sheng

AU - Ko, Yi Ching

AU - Hsu, Hsiang Hao

AU - Tseng, Fan Gang

PY - 2019

Y1 - 2019

N2 - In this research, we demonstrate successful glomerular cells cultivation outside co-shelled alginate tubes rather than cells cultivation inside the alginate tubes to reduce the interference of growth space on cell proliferation and differentiation. Podocytes were seeded on the outerior of an alginate tube by a tube infiltration method into a cell culturing site, and cells were successfully attached on the outer of the alginate tube and formed tubular structures instead of a cellular microfiber tube after 7 days cultivation. The reconstructed glomerular tube will be used as an in-vitro model for drug testing, and also the first step toward re-establishing a fully functional artificial glomerular.

AB - In this research, we demonstrate successful glomerular cells cultivation outside co-shelled alginate tubes rather than cells cultivation inside the alginate tubes to reduce the interference of growth space on cell proliferation and differentiation. Podocytes were seeded on the outerior of an alginate tube by a tube infiltration method into a cell culturing site, and cells were successfully attached on the outer of the alginate tube and formed tubular structures instead of a cellular microfiber tube after 7 days cultivation. The reconstructed glomerular tube will be used as an in-vitro model for drug testing, and also the first step toward re-establishing a fully functional artificial glomerular.

KW - Alginate

KW - Co-shelled tube

KW - Podocyte

KW - Tissue Engineering

UR - https://www.scopus.com/pages/publications/85094960407

M3 - 会议稿件

AN - SCOPUS:85094960407

T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

SP - 1596

EP - 1597

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

PB - Chemical and Biological Microsystems Society

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Y2 - 27 October 2019 through 31 October 2019

ER -

Chen YY, Chen JW, Chen JS, Ko YC, Hsu HH, Tseng FG. Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society. 2019. p. 1596-1597. (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

Guiding 3d podocyte cells cultivation on the outer coaxial alginate tubes to make glomerular on the chip

Abstract

Publication series

Conference

Bibliographical note

Keywords

Other files and links

Fingerprint

Cite this