TY - JOUR
T1 - Heme oxygenase-1 mediates the anti-inflammatory effect of curcumin within LPS-stimulated human monocytes
AU - Hsu, Hsien Yeh
AU - Chu, Li Chieh
AU - Hua, Kuo Feng
AU - Chao, Louis Kuoping
PY - 2008/6
Y1 - 2008/6
N2 - Curcumin, a polyphenolic compound derived from plant, regulates heme oxygenase (HO-1) expression within certain cell types; however, the Curcumin-mediated signal transduction in the regulation of HO-1 expression within human monocytes/macrophages is unclear. Herein, we show that Curcumin dose dependently induced HO-1 expression and HO-1 activity through the activation of PKCα, PKCδ/ ERK1/2, p38α, and PI3-kinase, In addition, H2O2 release is essential for Curcumin-mediated ERK1/2 and p38 phosphorylation and HO-1 expression. Further, Curcumin inhibited LPS-induced IL-1 and IL-6 secretion and blockage of HO-1 expression/activity by HO-1 siRNA or HO-1 inhibitor, SnPP reversed the inhibitory effects of Curcumin on cytokines secretion, HO-1 over-expression produced the same inhibitory effects of Curcumin on IL-1 secretion. Collectively, our results suggest that Curcumin inhibits cytokines secretion within LPS-stimulated monocytes through a mechanism that involves the action of HO-1.
AB - Curcumin, a polyphenolic compound derived from plant, regulates heme oxygenase (HO-1) expression within certain cell types; however, the Curcumin-mediated signal transduction in the regulation of HO-1 expression within human monocytes/macrophages is unclear. Herein, we show that Curcumin dose dependently induced HO-1 expression and HO-1 activity through the activation of PKCα, PKCδ/ ERK1/2, p38α, and PI3-kinase, In addition, H2O2 release is essential for Curcumin-mediated ERK1/2 and p38 phosphorylation and HO-1 expression. Further, Curcumin inhibited LPS-induced IL-1 and IL-6 secretion and blockage of HO-1 expression/activity by HO-1 siRNA or HO-1 inhibitor, SnPP reversed the inhibitory effects of Curcumin on cytokines secretion, HO-1 over-expression produced the same inhibitory effects of Curcumin on IL-1 secretion. Collectively, our results suggest that Curcumin inhibits cytokines secretion within LPS-stimulated monocytes through a mechanism that involves the action of HO-1.
UR - http://www.scopus.com/inward/record.url?scp=42949162968&partnerID=8YFLogxK
U2 - 10.1002/jcp.21206
DO - 10.1002/jcp.21206
M3 - 文章
C2 - 18357586
AN - SCOPUS:42949162968
SN - 0021-9541
VL - 215
SP - 603
EP - 612
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 3
ER -