High-density 3D nanostructured pillar arrays of Surface Enhanced Raman Scattering (SERS) biosensor for single bacteria detection by local electrokinetic trapping

Jen Kuei Wu, Chun Wei Lee, Teng Feng Kuo, Hwan You Chang, Fan Gang Tseng*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Early diagnosis of bacterial infection is the key for improved survival by prompt and adequate antibiotic in the first few hours (2-3 hours) of infection. In this work, we demonstrate a possibility to rapid detection several pathogenic bacteria from blood plasma in 30 mins. Using modified gold nanoparticle enlarge Surface Enhanced Raman Scattering (SERS) on a High-Density 3D nanostructured Au/Si-pillar arrays platform (10-12 array/μm2) combined with AC electroosmoic (ACEO) trapping. ACEO and corresponding electrostatic force contributed to the binding affinity and reduce the diffusion time. The limit of detection could be lowered to 1-5 cells/ml detected by SERS measurements. The dynamic range of the sensor system ranged from 102-106 cells/mL. The results showed that the portable biochip system has great potential as a device for single-particle or possibly even single-organism detection.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1484-1486
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 10 201331 10 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • AC electroosmoic (ACEO) trapping
  • Au/Si nanopillar arrays
  • SERS biosensor

Fingerprint

Dive into the research topics of 'High-density 3D nanostructured pillar arrays of Surface Enhanced Raman Scattering (SERS) biosensor for single bacteria detection by local electrokinetic trapping'. Together they form a unique fingerprint.

Cite this