TY - JOUR
T1 - High-speed mass analysis of whole erythrocytes by charge-detection quadrupole ion trap mass spectrometry
AU - Nie, Zongxiu
AU - Cui, Fenping
AU - Tzeng, Yan Kai
AU - Chang, Huan Cheng
AU - Chu, Minglee
AU - Lin, Huan Chang
AU - Chen, Chung Hsuan
AU - Lin, Hsin Hung
AU - Yu, Alice L.
PY - 2007/10/1
Y1 - 2007/10/1
N2 - Herein, we report an application of charge-detection quadrupole ion trap mass spectrometry to the measurement of total dry masses of mammalian and poultry erythrocytes evaporated/ionized by laser-induced acoustic desorption. The method is rapid and widely applicable. Eight different types of red blood cells (RBCs) have been successfully analyzed, including those of human, goat, cow, mouse, pig, and chicken. The measured mean masses (weights per corpuscle) range from 0.58 × 1013 Da (9.6 pg) of goat RBCs to 2.80 × 1013 Da (46.5 pg) of chicken RBCs. The total dry weights determined for human RBCs from a healthy male adult, a patient with iron-deficiency anemia, and a patient with thalassemia are 34.8, 28.8, and 20.6 pg, respectively. These weights, except that of thalassemia, are all ∼10% higher than their corresponding mean corpuscular hemoglobin values determined by a commercial automated hematology analyzer. The mass distribution profiles of the cells are all near-Gaussian, with a standard deviation of 15% for the normal human RBCs. The deviation increases significantly to 20% for RBCs with thalassemia characteristics and 27% for RBCs with iron-deficiency anemia characteristics. All the observations are in accord with their corresponding mean corpuscular volume measurements, indicating an increase in anisocytosis (variation in RBC size) in the anemic samples. Our results suggest a broad and promising application of this new technology to high-speed mass analysis of RBCs and other biological whole cells as well.
AB - Herein, we report an application of charge-detection quadrupole ion trap mass spectrometry to the measurement of total dry masses of mammalian and poultry erythrocytes evaporated/ionized by laser-induced acoustic desorption. The method is rapid and widely applicable. Eight different types of red blood cells (RBCs) have been successfully analyzed, including those of human, goat, cow, mouse, pig, and chicken. The measured mean masses (weights per corpuscle) range from 0.58 × 1013 Da (9.6 pg) of goat RBCs to 2.80 × 1013 Da (46.5 pg) of chicken RBCs. The total dry weights determined for human RBCs from a healthy male adult, a patient with iron-deficiency anemia, and a patient with thalassemia are 34.8, 28.8, and 20.6 pg, respectively. These weights, except that of thalassemia, are all ∼10% higher than their corresponding mean corpuscular hemoglobin values determined by a commercial automated hematology analyzer. The mass distribution profiles of the cells are all near-Gaussian, with a standard deviation of 15% for the normal human RBCs. The deviation increases significantly to 20% for RBCs with thalassemia characteristics and 27% for RBCs with iron-deficiency anemia characteristics. All the observations are in accord with their corresponding mean corpuscular volume measurements, indicating an increase in anisocytosis (variation in RBC size) in the anemic samples. Our results suggest a broad and promising application of this new technology to high-speed mass analysis of RBCs and other biological whole cells as well.
UR - https://www.scopus.com/pages/publications/35349030197
U2 - 10.1021/ac071207e
DO - 10.1021/ac071207e
M3 - 文章
C2 - 17784735
AN - SCOPUS:35349030197
SN - 0003-2700
VL - 79
SP - 7401
EP - 7407
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -