High-throughput cells separation and circulating tumor cells (CTCs) enrichment from whole blood by hydrodynamic and inertial force

Yu Cheng Chang*, Horas Cendana Tseng, Ren Guei Wu, Hwan You Chang, Fan Gang Tseng

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

To solve clogging in microchannel and low separation rate in microfluidic devices that containing high concentration whole blood cells (4.3x106 cells/μl) with the remaining <1% consisting peripheral blood leukocytes and platelets.1 In this work we introduce a like-biomimetic structure to separate red blood cells from whole blood and to enrich leukocytes (white blood cells) through the elastic properties of blood cell by the interaction of inertial force, shear lift, and wall crowding effect in a continuous flow system. A high separation efficiency (∼84%) and high throughput flow rate (0.4ml/min) was obtained in this microfluidic chip.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages266-267
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 09 10 201613 10 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period09/10/1613/10/16

Keywords

  • Circulating tumor cell
  • Erythrocytes separation
  • Sorting

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