Histone H1 Vaccine Therapy for Overcoming Acute Rejection in Experimental Organ Transplantation

T. Nakano; K. Ono; S. Goto; C. Y. Lai; L. W. Hsu; S. Kawamoto; Y. C. Lin; Y. H. Kao; K. C. Chiang; N. Ohmori; T. Goto; S. Sato; B. Jawan; Y. F. Cheng; C. L. Chen

doi:10.1016/j.transproceed.2006.10.078

Histone H1 Vaccine Therapy for Overcoming Acute Rejection in Experimental Organ Transplantation

T. Nakano, K. Ono, S. Goto, C. Y. Lai, L. W. Hsu, S. Kawamoto, Y. C. Lin, Y. H. Kao, K. C. Chiang, N. Ohmori, T. Goto, S. Sato, B. Jawan, Y. F. Cheng, C. L. Chen^*

^*Corresponding author for this work

Chang Gung Memorial Hospital

Research output: Contribution to journal › Journal Article › peer-review

3 Scopus citations

Abstract

Objective: In a rat tolerogenic orthotopic liver transplantation (OLT) model, the recipient serum (post-OLT serum) shows strong immunosuppressive activity. In our previous reports, we suggested that autoreactive antibody (Ab) against histone H1 is a major immunosuppressive factor in this serum. The present study sought to determine whether up-regulation of anti-histone H1 Ab by histone H1 vaccination led to tolerance. Materials and Methods: Using mixed lymphocyte reactions (MLR) and heterotopic heart transplantations (HHT), the alloreactive T-cell responses and allograft survivals of histone H1-immunized rats were compared with those of control rats. Cytokine and cellular profiles were determined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. Results: The alloreactive T-cell response of histone H1-immunized rats was significantly lower than that of control rats, although there was no difference in nonspecific T-cell activation between the 2 groups. The allograft survival of histone H1-immunized rats was significantly prolonged after HHT. The major histocompatibility complex (MHC) class II and CD25 molecules of histone H1-immunized rats were significantly down-regulated compared with those of control rats. Moreover, the serum cytokine profile was modified by the immunization with histone H1. Conclusions: These results suggest that histone H1 vaccination of transplant recipients leads to the production of immunosuppressive factors and the modification of cytokine/cellular profiles.

Original language	English
Pages (from-to)	3247-3248
Number of pages	2
Journal	Transplantation Proceedings
Volume	38
Issue number	10
DOIs	https://doi.org/10.1016/j.transproceed.2006.10.078
State	Published - 12 2006
Externally published	Yes

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Nakano, T., Ono, K., Goto, S., Lai, C. Y., Hsu, L. W., Kawamoto, S., Lin, Y. C., Kao, Y. H., Chiang, K. C., Ohmori, N., Goto, T., Sato, S., Jawan, B., Cheng, Y. F., & Chen, C. L. (2006). Histone H1 Vaccine Therapy for Overcoming Acute Rejection in Experimental Organ Transplantation. Transplantation Proceedings, 38(10), 3247-3248. https://doi.org/10.1016/j.transproceed.2006.10.078

@article{aa1dc59379ac42e18bf3344dfab9b569,

title = "Histone H1 Vaccine Therapy for Overcoming Acute Rejection in Experimental Organ Transplantation",

abstract = "Objective: In a rat tolerogenic orthotopic liver transplantation (OLT) model, the recipient serum (post-OLT serum) shows strong immunosuppressive activity. In our previous reports, we suggested that autoreactive antibody (Ab) against histone H1 is a major immunosuppressive factor in this serum. The present study sought to determine whether up-regulation of anti-histone H1 Ab by histone H1 vaccination led to tolerance. Materials and Methods: Using mixed lymphocyte reactions (MLR) and heterotopic heart transplantations (HHT), the alloreactive T-cell responses and allograft survivals of histone H1-immunized rats were compared with those of control rats. Cytokine and cellular profiles were determined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. Results: The alloreactive T-cell response of histone H1-immunized rats was significantly lower than that of control rats, although there was no difference in nonspecific T-cell activation between the 2 groups. The allograft survival of histone H1-immunized rats was significantly prolonged after HHT. The major histocompatibility complex (MHC) class II and CD25 molecules of histone H1-immunized rats were significantly down-regulated compared with those of control rats. Moreover, the serum cytokine profile was modified by the immunization with histone H1. Conclusions: These results suggest that histone H1 vaccination of transplant recipients leads to the production of immunosuppressive factors and the modification of cytokine/cellular profiles.",

author = "T. Nakano and K. Ono and S. Goto and Lai, {C. Y.} and Hsu, {L. W.} and S. Kawamoto and Lin, {Y. C.} and Kao, {Y. H.} and Chiang, {K. C.} and N. Ohmori and T. Goto and S. Sato and B. Jawan and Cheng, {Y. F.} and Chen, {C. L.}",

year = "2006",

month = dec,

doi = "10.1016/j.transproceed.2006.10.078",

language = "英语",

volume = "38",

pages = "3247--3248",

journal = "Transplantation Proceedings",

issn = "0041-1345",

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TY - JOUR

T1 - Histone H1 Vaccine Therapy for Overcoming Acute Rejection in Experimental Organ Transplantation

AU - Nakano, T.

AU - Ono, K.

AU - Goto, S.

AU - Lai, C. Y.

AU - Hsu, L. W.

AU - Kawamoto, S.

AU - Lin, Y. C.

AU - Kao, Y. H.

AU - Chiang, K. C.

AU - Ohmori, N.

AU - Goto, T.

AU - Sato, S.

AU - Jawan, B.

AU - Cheng, Y. F.

AU - Chen, C. L.

PY - 2006/12

Y1 - 2006/12

N2 - Objective: In a rat tolerogenic orthotopic liver transplantation (OLT) model, the recipient serum (post-OLT serum) shows strong immunosuppressive activity. In our previous reports, we suggested that autoreactive antibody (Ab) against histone H1 is a major immunosuppressive factor in this serum. The present study sought to determine whether up-regulation of anti-histone H1 Ab by histone H1 vaccination led to tolerance. Materials and Methods: Using mixed lymphocyte reactions (MLR) and heterotopic heart transplantations (HHT), the alloreactive T-cell responses and allograft survivals of histone H1-immunized rats were compared with those of control rats. Cytokine and cellular profiles were determined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. Results: The alloreactive T-cell response of histone H1-immunized rats was significantly lower than that of control rats, although there was no difference in nonspecific T-cell activation between the 2 groups. The allograft survival of histone H1-immunized rats was significantly prolonged after HHT. The major histocompatibility complex (MHC) class II and CD25 molecules of histone H1-immunized rats were significantly down-regulated compared with those of control rats. Moreover, the serum cytokine profile was modified by the immunization with histone H1. Conclusions: These results suggest that histone H1 vaccination of transplant recipients leads to the production of immunosuppressive factors and the modification of cytokine/cellular profiles.

AB - Objective: In a rat tolerogenic orthotopic liver transplantation (OLT) model, the recipient serum (post-OLT serum) shows strong immunosuppressive activity. In our previous reports, we suggested that autoreactive antibody (Ab) against histone H1 is a major immunosuppressive factor in this serum. The present study sought to determine whether up-regulation of anti-histone H1 Ab by histone H1 vaccination led to tolerance. Materials and Methods: Using mixed lymphocyte reactions (MLR) and heterotopic heart transplantations (HHT), the alloreactive T-cell responses and allograft survivals of histone H1-immunized rats were compared with those of control rats. Cytokine and cellular profiles were determined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. Results: The alloreactive T-cell response of histone H1-immunized rats was significantly lower than that of control rats, although there was no difference in nonspecific T-cell activation between the 2 groups. The allograft survival of histone H1-immunized rats was significantly prolonged after HHT. The major histocompatibility complex (MHC) class II and CD25 molecules of histone H1-immunized rats were significantly down-regulated compared with those of control rats. Moreover, the serum cytokine profile was modified by the immunization with histone H1. Conclusions: These results suggest that histone H1 vaccination of transplant recipients leads to the production of immunosuppressive factors and the modification of cytokine/cellular profiles.

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U2 - 10.1016/j.transproceed.2006.10.078

DO - 10.1016/j.transproceed.2006.10.078

M3 - 文章

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AN - SCOPUS:33845401724

SN - 0041-1345

VL - 38

SP - 3247

EP - 3248

JO - Transplantation Proceedings

JF - Transplantation Proceedings

IS - 10

ER -

Histone H1 Vaccine Therapy for Overcoming Acute Rejection in Experimental Organ Transplantation

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