Homogeneous phenomenon of the graft when using different genotype characteristic of recipients/donors inliving donor liver transplantation

AIM: To investigate the evidence of homogeneous phenomenon on CYP3A5*3 MDR1-3435 and CYP3A4 *18 of the liver graft after living donor liver transplantation (LDLT). METHODS: We identified the proportional change of the CYP3A5 * 3, MDR1-3435 and CYP3A4 * 18 from the peripheral blood mononuclear cell of 41 pairs recipient/ donor with different genotype polymorphisms and 119 liver graft biopsy samples used with the pyrosequencing technique after LDLT. Polymerase chain reaction/ligase detection reaction assay and restriction fragment length polymorphism was employed for genotyping the CYP3A5 * 3 and CYP3A4 * 18 single nucleotide polymorphisms (SNPs). All of the recipients and donors expressed with the similar SNP genotype of CYP3A5 * 3, MDR1-3435 or CYP3A4 * 18 were excluded.RESULTS: The final genetic polymorphisms of the liver graft biopsy samples of CYP3A5 * 3, MDR1-3435 and CYP3A4 * 18 was predominated depends on the donor with restriction fragment length polymorphism and seems tobe less related to the recipient. The proportional changes of G to A alleles of the 119 samples of CYP3A5 * 3 (included A > A/G, A/G > A, A/G > G, G > A, G > A/G and A > G), C to T alleles of the 108 samples of MDR1-3435 (included C > C/T, C/T > C, C/T > T, T > C/T and T > C), and T to C alleles of 15 samples of CYP3A4 * 18 (included T/C > T and T > C/T) were significant different between the recipients and the liver graft biopsy samples (P<0.0001) and less difference when compared with the donors in the pyrosequencing analysis after LDLT. CONCLUSION: The CYP3A5 *3, MDR1-3435 and CYP3A4 * 18 of the recipient could be modified by the donor so-called homogenous phenomenon when the recipient's blood drained into the liver graft.