Honokiol and magnolol increased hippocampal acetylcholine release in freely-moving rats

Honokiol and magnolol, phenolic compounds isolated from the stem bark of Magnolia offlcinalis, have been demonstrated to increase choline acctyltransferase activity, inhibit acetylcholinesterase, promote potassium-induced acetylcholine release and exhibit neurotrophic function in in vitro studies. The objective of the present study was to determine the effect of these compounds on hippocampal acetylcholine release in conscious, freely-moving rats. 10^-4 M-10^-6 M of honokiol or magnolol was perfused into rat hippocampus via a dialysis probe. The results showed that at 10^-4 M concentration, honokiol and magnolol markedly increased extracellular acetylcholine release to 165.5 ±5.78% and 237.83 ±9.47% of the basal level, respectively. However, lower concentrations of either compounds failed to elicit significant acetylcholine release. This result suggests that a high dose of honokiol or magnolol may enhance in vivo hippocampal acetylcholine release.