Hypermethylation of the CpG islands in the promoter region flanking GSTP1 gene is a potential plasma DNA biomarker for detecting prostate carcinoma

Cheng Keng Chuang, Da Chang Chu*, Ron Dar Tzou, Shu I. Liou, Ju Hsin Chia, Chien Feng Sun

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

39 Scopus citations


Objectives: To investigate the possibility of identifying DNA hypermethylation in the circulation of prostate cancer patients. Methods: Plasma DNA samples were extracted from 36 prostate cancer patients and 27 benign prostate hyperplasia (BPH) cases. After extensive methylation-sensitive restriction enzyme digestion, the DNA samples were subjected to the real-time quantitative PCR amplification. Dissociation curve analysis was applied to determine if hypermethylation occurred in the promoter region flanking the GSTP1 gene, a well-documented epigenetic event among prostate cancer cells, in these plasma DNA samples. Results: 11 of 36 prostate cancer patients showed positive peak pattern, indicating methylation changes occurred. Concordant data were obtained from the corresponding paraffin-embedded tissue samples available from the Tumor Bank. Twenty-five of the 27 BPH cases showed negative results, suggesting no methylation changes happened in the CpG islands in these cases. Conclusions: We have successfully identified prostate cancer genome hypermethylation in the peripheral circulation in prostate cancer patients with this protocol. This method can effectively distinguish BPH from prostate neoplasm. Although a larger number of samples are necessary to validate the capability of the protocol in practice, using plasma DNA sample is an ideal non-invasive approach for prostate neoplasm detection.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalCancer Detection and Prevention
Issue number1
StatePublished - 2007
Externally publishedYes


  • Benign prostate hyperplasia
  • Circulating DNA
  • DNA extraction
  • Epigenetic alterations
  • GSTP1 gene
  • Gleason score
  • Hypermethylation
  • Methylation-sensitive real-time quantitative PCR
  • Paraffin-embedded tissue blocks
  • Plasma DNA
  • Prostate cancer
  • Spectrofluorometry


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