Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

Z. B. Sun; J. W. Wang; H. Xiao; Q. S. Zhang; W. S. Kan; F. B. Mo; S. Hu; S. N. Ye

doi:10.1007/s00198-014-2809-z

Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

Z. B. Sun, J. W. Wang, H. Xiao, Q. S. Zhang, W. S. Kan, F. B. Mo, S. Hu^*, S. N. Ye

^*Corresponding author for this work

Huazhong University of Science and Technology

Research output: Contribution to journal › Journal Article › peer-review

57 Scopus citations

Abstract

Summary: In this study, we found out a previously undefined function of icariin which restored the dynamic balance between osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) in patients with osteonecrosis of femoral head (ONFH) via ABCB1-promoter demethylation. These findings provided important information regarding potential implication of icariin targeting epigenetic changes for the treatment of steroid -associated ONFH.

Introduction: Here, we investigated whether icariin can also exert a beneficial role in the reactivation of MSCs in the patients with steroid-associated ONFH via ABCB1-promoter demethylation.

Methods: Bone marrow was collected from the proximal femur in patients with steroid-associated ONFH (n = 20) and patients with new femoral neck fractures (n = 22), and then MSCs were isolated. We investigated cell viability, intracellular reactive oxygen species (ROS) level, mitochondrial membrane potential (MMP), P-glycoprotein (P-gp) activity, the transcript levels of ABCB1 and oxidative stress-related genes, methylation extent at CpG islands of ABCB1 promoter, and osteogenic and adipogenic differentiation ability of MSCs from the femoral neck fractures group and from the steroid-associated ONFH group treated with or without icariin.

Results: We observed that MSCs from the steroid-associated ONFH group showed reduced proliferation ability, elevated ROS level, depressed MMP, weakened osteogenesis, and enhanced adipogenesis while low P-gp activity, transcription level of ABCB1, and oxidative stress-related genes as well as aberrant CpG islands hypermethylation of ABCB1 were also noted in steroid-associated ONFH group. Treatment with icariin obviously induced de novo P-gp expression, decreased oxidative stress, and promoted osteogenesis.

Conclusion: Icariin may be a potential drug targeting epigenetic changes for the treatment of steroid-associated ONFH.

Original language	English
Pages (from-to)	187-197
Number of pages	11
Journal	Osteoporosis International
Volume	26
Issue number	1
DOIs	https://doi.org/10.1007/s00198-014-2809-z
State	Published - 01 2014
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014, International Osteoporosis Foundation and National Osteoporosis Foundation.

Keywords

ABCB1
Hypermethylation
Icariin
Steroid-associated osteonecrosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00198-014-2809-z

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@article{fe544ec2fe7a4474a467b2b0151e7c23,

title = "Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study",

abstract = "Summary: In this study, we found out a previously undefined function of icariin which restored the dynamic balance between osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) in patients with osteonecrosis of femoral head (ONFH) via ABCB1-promoter demethylation. These findings provided important information regarding potential implication of icariin targeting epigenetic changes for the treatment of steroid -associated ONFH.Introduction: Here, we investigated whether icariin can also exert a beneficial role in the reactivation of MSCs in the patients with steroid-associated ONFH via ABCB1-promoter demethylation.Methods: Bone marrow was collected from the proximal femur in patients with steroid-associated ONFH (n = 20) and patients with new femoral neck fractures (n = 22), and then MSCs were isolated. We investigated cell viability, intracellular reactive oxygen species (ROS) level, mitochondrial membrane potential (MMP), P-glycoprotein (P-gp) activity, the transcript levels of ABCB1 and oxidative stress-related genes, methylation extent at CpG islands of ABCB1 promoter, and osteogenic and adipogenic differentiation ability of MSCs from the femoral neck fractures group and from the steroid-associated ONFH group treated with or without icariin.Results: We observed that MSCs from the steroid-associated ONFH group showed reduced proliferation ability, elevated ROS level, depressed MMP, weakened osteogenesis, and enhanced adipogenesis while low P-gp activity, transcription level of ABCB1, and oxidative stress-related genes as well as aberrant CpG islands hypermethylation of ABCB1 were also noted in steroid-associated ONFH group. Treatment with icariin obviously induced de novo P-gp expression, decreased oxidative stress, and promoted osteogenesis.Conclusion: Icariin may be a potential drug targeting epigenetic changes for the treatment of steroid-associated ONFH.",

keywords = "ABCB1, Hypermethylation, Icariin, Steroid-associated osteonecrosis",

author = "Sun, {Z. B.} and Wang, {J. W.} and H. Xiao and Zhang, {Q. S.} and Kan, {W. S.} and Mo, {F. B.} and S. Hu and Ye, {S. N.}",

note = "Publisher Copyright: {\textcopyright} 2014, International Osteoporosis Foundation and National Osteoporosis Foundation.",

year = "2014",

month = jan,

doi = "10.1007/s00198-014-2809-z",

language = "英语",

volume = "26",

pages = "187--197",

journal = "Osteoporosis International",

issn = "0937-941X",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "1",

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T1 - Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation

T2 - a preliminary study

AU - Sun, Z. B.

AU - Wang, J. W.

AU - Xiao, H.

AU - Zhang, Q. S.

AU - Kan, W. S.

AU - Mo, F. B.

AU - Hu, S.

AU - Ye, S. N.

PY - 2014/1

Y1 - 2014/1

N2 - Summary: In this study, we found out a previously undefined function of icariin which restored the dynamic balance between osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) in patients with osteonecrosis of femoral head (ONFH) via ABCB1-promoter demethylation. These findings provided important information regarding potential implication of icariin targeting epigenetic changes for the treatment of steroid -associated ONFH.Introduction: Here, we investigated whether icariin can also exert a beneficial role in the reactivation of MSCs in the patients with steroid-associated ONFH via ABCB1-promoter demethylation.Methods: Bone marrow was collected from the proximal femur in patients with steroid-associated ONFH (n = 20) and patients with new femoral neck fractures (n = 22), and then MSCs were isolated. We investigated cell viability, intracellular reactive oxygen species (ROS) level, mitochondrial membrane potential (MMP), P-glycoprotein (P-gp) activity, the transcript levels of ABCB1 and oxidative stress-related genes, methylation extent at CpG islands of ABCB1 promoter, and osteogenic and adipogenic differentiation ability of MSCs from the femoral neck fractures group and from the steroid-associated ONFH group treated with or without icariin.Results: We observed that MSCs from the steroid-associated ONFH group showed reduced proliferation ability, elevated ROS level, depressed MMP, weakened osteogenesis, and enhanced adipogenesis while low P-gp activity, transcription level of ABCB1, and oxidative stress-related genes as well as aberrant CpG islands hypermethylation of ABCB1 were also noted in steroid-associated ONFH group. Treatment with icariin obviously induced de novo P-gp expression, decreased oxidative stress, and promoted osteogenesis.Conclusion: Icariin may be a potential drug targeting epigenetic changes for the treatment of steroid-associated ONFH.

AB - Summary: In this study, we found out a previously undefined function of icariin which restored the dynamic balance between osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) in patients with osteonecrosis of femoral head (ONFH) via ABCB1-promoter demethylation. These findings provided important information regarding potential implication of icariin targeting epigenetic changes for the treatment of steroid -associated ONFH.Introduction: Here, we investigated whether icariin can also exert a beneficial role in the reactivation of MSCs in the patients with steroid-associated ONFH via ABCB1-promoter demethylation.Methods: Bone marrow was collected from the proximal femur in patients with steroid-associated ONFH (n = 20) and patients with new femoral neck fractures (n = 22), and then MSCs were isolated. We investigated cell viability, intracellular reactive oxygen species (ROS) level, mitochondrial membrane potential (MMP), P-glycoprotein (P-gp) activity, the transcript levels of ABCB1 and oxidative stress-related genes, methylation extent at CpG islands of ABCB1 promoter, and osteogenic and adipogenic differentiation ability of MSCs from the femoral neck fractures group and from the steroid-associated ONFH group treated with or without icariin.Results: We observed that MSCs from the steroid-associated ONFH group showed reduced proliferation ability, elevated ROS level, depressed MMP, weakened osteogenesis, and enhanced adipogenesis while low P-gp activity, transcription level of ABCB1, and oxidative stress-related genes as well as aberrant CpG islands hypermethylation of ABCB1 were also noted in steroid-associated ONFH group. Treatment with icariin obviously induced de novo P-gp expression, decreased oxidative stress, and promoted osteogenesis.Conclusion: Icariin may be a potential drug targeting epigenetic changes for the treatment of steroid-associated ONFH.

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KW - Steroid-associated osteonecrosis

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DO - 10.1007/s00198-014-2809-z

M3 - 文章

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AN - SCOPUS:84939893339

SN - 0937-941X

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JO - Osteoporosis International

JF - Osteoporosis International

IS - 1

ER -

Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

Abstract

Bibliographical note

Keywords

UN SDGs

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