Identification and characterization of chlorine-containing briaranes from a cultured octocoral briareum excavatum (briareidae)

Six chlorine-containing briaranes, including two new compounds, briarenols M (1) and N (2), as well as four known metabolites solenolide E (3), briarenolide R (4), excavatolide A (5), and briaexcavatolide F (6), have been isolated from a cultured octocoral identified as Briareum excavatum. The structures of 1 and 2 were established by spectroscopic methods and the absolute configurations of 5 and 6 were confirmed by single-crystal X-ray diffraction analysis. These compounds were subjected into the inhibitory effects on fMLF/CB stimulated superoxide anion generation and elastase release in human neutrophils.