Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

Yi Chyi Lai; Ging Ting Lin; Shu Li Yang; Hwan You Chang; Hwei Ling Peng

doi:10.1016/S0378-1097(02)01120-5

Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

Yi Chyi Lai, Ging Ting Lin, Shu Li Yang, Hwan You Chang, Hwei Ling Peng^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

20 Scopus citations

Abstract

A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.

Original language	English
Pages (from-to)	121-126
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	218
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(02)01120-5
State	Published - 21 01 2003
Externally published	Yes

Keywords

Histidine kinase
Klebsiella gene
Response regulator
Two-component system

Access to Document

10.1016/S0378-1097(02)01120-5

Cite this

@article{77d79882ab6745ba8342d5e966c46937,

title = "Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43",

abstract = "A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.",

keywords = "Histidine kinase, Klebsiella gene, Response regulator, Two-component system",

author = "Lai, {Yi Chyi} and Lin, {Ging Ting} and Yang, {Shu Li} and Chang, {Hwan You} and Peng, {Hwei Ling}",

year = "2003",

month = jan,

day = "21",

doi = "10.1016/S0378-1097(02)01120-5",

language = "英语",

volume = "218",

pages = "121--126",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

AU - Lai, Yi Chyi

AU - Lin, Ging Ting

AU - Yang, Shu Li

AU - Chang, Hwan You

AU - Peng, Hwei Ling

PY - 2003/1/21

Y1 - 2003/1/21

N2 - A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.

AB - A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.

KW - Histidine kinase

KW - Klebsiella gene

KW - Response regulator

KW - Two-component system

UR - http://www.scopus.com/inward/record.url?scp=0037458144&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(02)01120-5

DO - 10.1016/S0378-1097(02)01120-5

M3 - 文章

C2 - 12583907

AN - SCOPUS:0037458144

SN - 0378-1097

VL - 218

SP - 121

EP - 126

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this