Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

Yi Chyi Lai; Ging Ting Lin; Shu Li Yang; Hwan You Chang; Hwei Ling Peng

doi:10.1016/S0378-1097(02)01120-5

Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

Yi Chyi Lai
, Ging Ting Lin
, Shu Li Yang
, Hwan You Chang
, Hwei Ling Peng^*

^*Corresponding author for this work

National Tsing Hua University
National Yang Ming Chiao Tung University

Research output: Contribution to journal › Journal Article › peer-review

21 Scopus citations

Abstract

A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.

Original language	English
Pages (from-to)	121-126
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	218
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(02)01120-5
State	Published - 21 01 2003
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Histidine kinase
Klebsiella gene
Response regulator
Two-component system

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10.1016/S0378-1097(02)01120-5

Cite this

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title = "Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43",

abstract = "A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.",

keywords = "Histidine kinase, Klebsiella gene, Response regulator, Two-component system",

author = "Lai, \{Yi Chyi\} and Lin, \{Ging Ting\} and Yang, \{Shu Li\} and Chang, \{Hwan You\} and Peng, \{Hwei Ling\}",

year = "2003",

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doi = "10.1016/S0378-1097(02)01120-5",

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T1 - Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

AU - Lai, Yi Chyi

AU - Lin, Ging Ting

AU - Yang, Shu Li

AU - Chang, Hwan You

AU - Peng, Hwei Ling

PY - 2003/1/21

Y1 - 2003/1/21

N2 - A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.

AB - A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.

KW - Histidine kinase

KW - Klebsiella gene

KW - Response regulator

KW - Two-component system

UR - https://www.scopus.com/pages/publications/0037458144

U2 - 10.1016/S0378-1097(02)01120-5

DO - 10.1016/S0378-1097(02)01120-5

M3 - 文章

C2 - 12583907

AN - SCOPUS:0037458144

SN - 0378-1097

VL - 218

SP - 121

EP - 126

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

Abstract

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Keywords

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