TY - JOUR
T1 - Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay
AU - Kao, Po Min
AU - Tung, Min Che
AU - Hsu, Bing Mu
AU - Chiu, Yi Chou
AU - She, Cheng Yu
AU - Shen, Shu Min
AU - Huang, Yu Li
AU - Huang, Wen Chien
PY - 2013/9
Y1 - 2013/9
N2 - In this study, a SYBR green quantitative real-time PCR was developed to quantify and detect the Legionella spp. in various environmental water samples. The water samples were taken from watershed, water treatment plant, and thermal spring area in Taiwan. Legionella was detected in 13.6 % (24/176), and the detection rate for river water, raw drinking water, and thermal spring water was 10, 21.4, and 16.6 %, respectively. Using real-time PCR, concentration of Legionella spp. in detected samples ranged between 9.75 × 104 and 3.47 × 105 cells/L in river water, 6.92 × 104 and 4.29 × 105 cells/L in raw drinking water, and 5.71 × 104 and 2.12 × 106 cells/L for thermal spring water samples. The identified species included Legionella pneumophila (20.8 %), Legionella jordanis (4.2 %), Legionella nautarum (4.2 %), Legionella sp. (4.2 %), and uncultured Legionella sp. (66.6 %). The presence of L. pneumophila in aquatic environments suggested a potential public health threat that must be further examined.
AB - In this study, a SYBR green quantitative real-time PCR was developed to quantify and detect the Legionella spp. in various environmental water samples. The water samples were taken from watershed, water treatment plant, and thermal spring area in Taiwan. Legionella was detected in 13.6 % (24/176), and the detection rate for river water, raw drinking water, and thermal spring water was 10, 21.4, and 16.6 %, respectively. Using real-time PCR, concentration of Legionella spp. in detected samples ranged between 9.75 × 104 and 3.47 × 105 cells/L in river water, 6.92 × 104 and 4.29 × 105 cells/L in raw drinking water, and 5.71 × 104 and 2.12 × 106 cells/L for thermal spring water samples. The identified species included Legionella pneumophila (20.8 %), Legionella jordanis (4.2 %), Legionella nautarum (4.2 %), Legionella sp. (4.2 %), and uncultured Legionella sp. (66.6 %). The presence of L. pneumophila in aquatic environments suggested a potential public health threat that must be further examined.
KW - Legionella pneumophila
KW - Legionella spp.
KW - Real-time PCR
KW - Thermal spring
KW - Water treatment plant
KW - Watershed
UR - http://www.scopus.com/inward/record.url?scp=84880783000&partnerID=8YFLogxK
U2 - 10.1007/s11356-013-1534-z
DO - 10.1007/s11356-013-1534-z
M3 - 文章
AN - SCOPUS:84880783000
SN - 0944-1344
VL - 20
SP - 6128
EP - 6137
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
IS - 9
ER -
