Abstract
Background: Group B Streptococcus (GBS) is an important invasive pathogen in neonates, pregnant women and the elderly. Serotype VI GBS, which has been rarely reported globally, has emerged as a significant pathogen in Asia. However, traditional serologic latex agglutination (LA) methods may fail to type isolates that lack of or low expression of CPS. Methods: A total of 104 GBS strains were analyzed by MALDI-TOF MS. Multiplex PCR and multilocus sequence typing (MLST) were also performed to confirm their strains. The protein markers were purified with gel electrophoresis and LC-column, followed by identification with nanoLC–MS/MS analysis. Results: Protein peak of 6251-Da was appeared in most (20/24, 92%) serotypes VI (94% ST-1 or single locus variant of ST-1), and protein peak of 6891-Da was appeared in most serotypes III (15/18, 83%) and Ib (19/23, 83%) strains. The protein peak of 6251-Da and 6891-Da were identified as CsbD family protein and UPF0337 protein gbs0600, respectively. Conclusions: The protein peak of 6251 Da may play a role of emergence of ST-1 clone, serotype VI GBS in central Taiwan and could be useful in rapid identifying invasive serotype VI from III isolates, which is hardly achieved by LA.
Original language | English |
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Pages (from-to) | 81-89 |
Number of pages | 9 |
Journal | Journal of Microbiology, Immunology and Infection |
Volume | 52 |
Issue number | 1 |
DOIs | |
State | Published - 02 2019 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2018
Keywords
- Group B Streptococcus
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- Multilocus sequence typing
- Sequence type