TY - JOUR
T1 - Identification of heat-shock protein 90 beta in Japanese encephalitis virus-induced secretion proteins
AU - Hung, Chun Yu
AU - Tsai, Meng Chieh
AU - Wu, Yi Ping
AU - Wang, Robert Y.L.
PY - 2011/12
Y1 - 2011/12
N2 - Five host cellular proteins were identified in the secretion medium from Japanese encephalitis virus (JEV)-infected baby hamster kidney-21 (BHK-21) cells, including three molecular chaperones: Hsp70, GRP78 and Hsp90. Hsp90 isoforms were characterized further. Hsp90α was observed to be retained inside the nuclei, whereas Hsp90β associated with virus particles during assembly and was released into the secretion medium upon JEV infection. The association of Hsp90β and viral E protein was demonstrated by using sucrose-density fractionation and Western blot analysis. Moreover, JEV viral RNA replication was not affected by treatment with geldanamycin, an Hsp90 inhibitor, but impaired virus infectivity that was determined by a plaqueforming assay. Our results show that Hsp90β, not Hsp90α, is present in the JEV-induced secretion medium and is required for JEV infectivity in BHK-21 cells.
AB - Five host cellular proteins were identified in the secretion medium from Japanese encephalitis virus (JEV)-infected baby hamster kidney-21 (BHK-21) cells, including three molecular chaperones: Hsp70, GRP78 and Hsp90. Hsp90 isoforms were characterized further. Hsp90α was observed to be retained inside the nuclei, whereas Hsp90β associated with virus particles during assembly and was released into the secretion medium upon JEV infection. The association of Hsp90β and viral E protein was demonstrated by using sucrose-density fractionation and Western blot analysis. Moreover, JEV viral RNA replication was not affected by treatment with geldanamycin, an Hsp90 inhibitor, but impaired virus infectivity that was determined by a plaqueforming assay. Our results show that Hsp90β, not Hsp90α, is present in the JEV-induced secretion medium and is required for JEV infectivity in BHK-21 cells.
UR - http://www.scopus.com/inward/record.url?scp=81255188968&partnerID=8YFLogxK
U2 - 10.1099/vir.0.033993-0
DO - 10.1099/vir.0.033993-0
M3 - 文章
C2 - 21813703
AN - SCOPUS:81255188968
SN - 0022-1317
VL - 92
SP - 2803
EP - 2809
JO - Journal of General Virology
JF - Journal of General Virology
IS - 12
ER -