TY - JOUR
T1 - Identification of IL-28B genotype modification in hepatocytes after living donor liver transplantation by laser capture microdissection and pyrosequencing analysis
AU - Chiu, King Wah
AU - Nakano, Toshiaki
AU - Chen, Kuang Den
AU - Hu, Tsung Hui
AU - Lin, Chih Che
AU - Hsu, Li Wen
AU - Chen, Chao Long
AU - Goto, Shigeru
N1 - Publisher Copyright:
© 2018 King-Wah Chiu et al.
PY - 2018
Y1 - 2018
N2 - The aim of this study is to elucidate the biogenetic modification of donor and recipient interleukin-28B (IL-28B) genotypes in liver graft biopsies after living donor liver transplantation (LDLT) for chronic hepatitis C virus- (HCV-) related, end-stage liver disease. Fifty liver graft biopsies were collected from recipients during LDLT treatment for HCV-related, end-stage liver disease. DNA was extracted from all 50 liver tissues, and the IL-28B single-nucleotide polymorphisms (SNPs) rs8099917 and rs12979860 were studied for allelic discrimination by real-time PCR analysis. Blood samples were obtained from donors and recipients on postoperative day 0 (POD0), POD7, and POD30. We randomly selected five liver biopsies and isolated the hepatocytes by laser capture microdissection (LCM) to evaluate genotype modifications resulting from LDLT. After LDLT, the IL-28B SNP rs8099917 was identified not only in the liver graft biopsies and donors' sera (TT = 41: 43; GT = 9: 5; GG = 0: 2), but also in liver graft biopsies and recipients' sera on POD0 (TT = 41: 44; GT = 9: 4; GG = 0: 2), POD7 (TT = 41: 30; GT = 9: 18; GG = 0: 2), and POD30 (TT = 41: 29; GT = 9: 19; GG = 0: 2). A significant difference was observed between the rs8099917 allele frequencies of liver graft biopsies and recipients' sera on POD30 (p=0.039). In addition, a significant difference was also noted between the rs12979860 allele frequencies of liver graft biopsies and donors' sera (CT = 49: 39; TT = 1: 10) (p=0.012) and of liver graft biopsies and recipients' sera on POD0 (CT = 49: 39; TT = 1: 11) (p=0.002), POD7 (CT = 49: 42; TT = 1: 8) (p=0.016), and POD30 (CT = 49: 41; TT = 1: 9) (p=0.008). This phenomenon was confirmed by pyrosequencing of hepatocytes isolated by LCM. Following LDLT, the TT-to-GT IL-28B genotype modification predominated in rs8099917, and the CC-to-CT modification predominated in rs12979860. In conclusion, these modified phenomena suggested that the selected donor with a predictable and favourable IL-28B genotype will not confer a benefit on the recipient in the living donor liver transplantation setting.
AB - The aim of this study is to elucidate the biogenetic modification of donor and recipient interleukin-28B (IL-28B) genotypes in liver graft biopsies after living donor liver transplantation (LDLT) for chronic hepatitis C virus- (HCV-) related, end-stage liver disease. Fifty liver graft biopsies were collected from recipients during LDLT treatment for HCV-related, end-stage liver disease. DNA was extracted from all 50 liver tissues, and the IL-28B single-nucleotide polymorphisms (SNPs) rs8099917 and rs12979860 were studied for allelic discrimination by real-time PCR analysis. Blood samples were obtained from donors and recipients on postoperative day 0 (POD0), POD7, and POD30. We randomly selected five liver biopsies and isolated the hepatocytes by laser capture microdissection (LCM) to evaluate genotype modifications resulting from LDLT. After LDLT, the IL-28B SNP rs8099917 was identified not only in the liver graft biopsies and donors' sera (TT = 41: 43; GT = 9: 5; GG = 0: 2), but also in liver graft biopsies and recipients' sera on POD0 (TT = 41: 44; GT = 9: 4; GG = 0: 2), POD7 (TT = 41: 30; GT = 9: 18; GG = 0: 2), and POD30 (TT = 41: 29; GT = 9: 19; GG = 0: 2). A significant difference was observed between the rs8099917 allele frequencies of liver graft biopsies and recipients' sera on POD30 (p=0.039). In addition, a significant difference was also noted between the rs12979860 allele frequencies of liver graft biopsies and donors' sera (CT = 49: 39; TT = 1: 10) (p=0.012) and of liver graft biopsies and recipients' sera on POD0 (CT = 49: 39; TT = 1: 11) (p=0.002), POD7 (CT = 49: 42; TT = 1: 8) (p=0.016), and POD30 (CT = 49: 41; TT = 1: 9) (p=0.008). This phenomenon was confirmed by pyrosequencing of hepatocytes isolated by LCM. Following LDLT, the TT-to-GT IL-28B genotype modification predominated in rs8099917, and the CC-to-CT modification predominated in rs12979860. In conclusion, these modified phenomena suggested that the selected donor with a predictable and favourable IL-28B genotype will not confer a benefit on the recipient in the living donor liver transplantation setting.
UR - http://www.scopus.com/inward/record.url?scp=85046285680&partnerID=8YFLogxK
U2 - 10.1155/2018/1826140
DO - 10.1155/2018/1826140
M3 - 文章
C2 - 29686997
AN - SCOPUS:85046285680
SN - 2314-6133
VL - 2018
JO - BioMed Research International
JF - BioMed Research International
M1 - 1826140
ER -
