Identification of novel genes that are differentially expressed in human colorectal carcinoma

Err Cheng Chan; Min Yong Lue; Kung Cheng Hsu; Hong Arh Fan

doi:10.1016/S0925-4439(98)00041-6

Identification of novel genes that are differentially expressed in human colorectal carcinoma

Err Cheng Chan^*
, Min Yong Lue
, Kung Cheng Hsu
, Hong Arh Fan

^*Corresponding author for this work

Chang Gung University
Chang Gung Memorial Hospital

Research output: Contribution to journal › Journal Article › peer-review

6 Scopus citations

Abstract

By using mRNA differential display technology, we have identified three cDNA clones, myl 3, myl 4, and myl 6, to show significant changes in expression between human colorectal tumor and paired normal tissue. Northern blot analysis indicated that clone myl 3 expression was elevated in normal tissue, and clone myl 4 expression was elevated in tumor tissue. Nucleotide sequence analysis revealed that clones myl 3 and myl 4 have not been previously identified. However, clone myl 6 appears to be the human homolog of the 3' end region of tissue inhibitor of metalloproteinase 3 (TIMP-3). Northern blot analysis showed that a 2.5 kb TIMP-3 transcript was expressed at a much higher level in normal tissue than the colorectal tumor. Copyright (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	200-204
Number of pages	5
Journal	Biochimica et Biophysica Acta - Molecular Basis of Disease
Volume	1407
Issue number	3
DOIs	https://doi.org/10.1016/S0925-4439(98)00041-6
State	Published - 30 09 1998
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Colorectal tumor
Reverse transcriptase-polymerase chain reaction
Tissue inhibitor of metalloproteinase 3
mRNA differential display

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10.1016/S0925-4439(98)00041-6

Cite this

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title = "Identification of novel genes that are differentially expressed in human colorectal carcinoma",

abstract = "By using mRNA differential display technology, we have identified three cDNA clones, myl 3, myl 4, and myl 6, to show significant changes in expression between human colorectal tumor and paired normal tissue. Northern blot analysis indicated that clone myl 3 expression was elevated in normal tissue, and clone myl 4 expression was elevated in tumor tissue. Nucleotide sequence analysis revealed that clones myl 3 and myl 4 have not been previously identified. However, clone myl 6 appears to be the human homolog of the 3' end region of tissue inhibitor of metalloproteinase 3 (TIMP-3). Northern blot analysis showed that a 2.5 kb TIMP-3 transcript was expressed at a much higher level in normal tissue than the colorectal tumor. Copyright (C) 1998 Elsevier Science B.V.",

keywords = "Colorectal tumor, Reverse transcriptase-polymerase chain reaction, Tissue inhibitor of metalloproteinase 3, mRNA differential display",

author = "Chan, \{Err Cheng\} and Lue, \{Min Yong\} and Hsu, \{Kung Cheng\} and Fan, \{Hong Arh\}",

year = "1998",

month = sep,

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doi = "10.1016/S0925-4439(98)00041-6",

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TY - JOUR

T1 - Identification of novel genes that are differentially expressed in human colorectal carcinoma

AU - Chan, Err Cheng

AU - Lue, Min Yong

AU - Hsu, Kung Cheng

AU - Fan, Hong Arh

PY - 1998/9/30

Y1 - 1998/9/30

N2 - By using mRNA differential display technology, we have identified three cDNA clones, myl 3, myl 4, and myl 6, to show significant changes in expression between human colorectal tumor and paired normal tissue. Northern blot analysis indicated that clone myl 3 expression was elevated in normal tissue, and clone myl 4 expression was elevated in tumor tissue. Nucleotide sequence analysis revealed that clones myl 3 and myl 4 have not been previously identified. However, clone myl 6 appears to be the human homolog of the 3' end region of tissue inhibitor of metalloproteinase 3 (TIMP-3). Northern blot analysis showed that a 2.5 kb TIMP-3 transcript was expressed at a much higher level in normal tissue than the colorectal tumor. Copyright (C) 1998 Elsevier Science B.V.

AB - By using mRNA differential display technology, we have identified three cDNA clones, myl 3, myl 4, and myl 6, to show significant changes in expression between human colorectal tumor and paired normal tissue. Northern blot analysis indicated that clone myl 3 expression was elevated in normal tissue, and clone myl 4 expression was elevated in tumor tissue. Nucleotide sequence analysis revealed that clones myl 3 and myl 4 have not been previously identified. However, clone myl 6 appears to be the human homolog of the 3' end region of tissue inhibitor of metalloproteinase 3 (TIMP-3). Northern blot analysis showed that a 2.5 kb TIMP-3 transcript was expressed at a much higher level in normal tissue than the colorectal tumor. Copyright (C) 1998 Elsevier Science B.V.

KW - Colorectal tumor

KW - Reverse transcriptase-polymerase chain reaction

KW - Tissue inhibitor of metalloproteinase 3

KW - mRNA differential display

UR - https://www.scopus.com/pages/publications/0032412603

U2 - 10.1016/S0925-4439(98)00041-6

DO - 10.1016/S0925-4439(98)00041-6

M3 - 文章

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AN - SCOPUS:0032412603

SN - 0925-4439

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JO - Biochimica et Biophysica Acta - Molecular Basis of Disease

JF - Biochimica et Biophysica Acta - Molecular Basis of Disease

IS - 3

ER -

Identification of novel genes that are differentially expressed in human colorectal carcinoma

Abstract

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Keywords

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