Identification of Scavenger Receptor Ligands

Claudine Neyen; Annette Pluddemann; Siamon Gordon

doi:10.1007/978-1-61779-139-0_3

Identification of Scavenger Receptor Ligands

Claudine Neyen, Annette Pluddemann, Siamon Gordon^*

^*Corresponding author for this work

University of Oxford

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Scavenger receptors (SRs) are structurally diverse but functionally related innate immune receptors involved in defence and clearance mechanisms. Their broad specificity relies on evolutionarily conserved pattern recognition domains which interact with a variety of microbial, apoptotic and modified self ligands. Studies of immune functions of SR-expressing cells require the identification of interaction partners for SRs. We have developed an ELISA-based method which allows for large-scale high-throughput screening of complex mixtures. The assay successfully identified bacterial and plasma ligands for macrophage scavenger receptor A and can be adapted to screen for novel exogenous or endogenous ligands for any immune receptor of interest.

Original language	English
Title of host publication	Immune Receptors
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	35-50
Number of pages	16
ISBN (Print)	9781617791383
DOIs	https://doi.org/10.1007/978-1-61779-139-0_3
State	Published - 2011
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	748
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.

Keywords

ELISA
Far Western Blot
Innate immunity
Interaction
Ligand
Macrophage
Receptor
Scavenger receptor
Screening

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10.1007/978-1-61779-139-0_3

Cite this

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abstract = "Scavenger receptors (SRs) are structurally diverse but functionally related innate immune receptors involved in defence and clearance mechanisms. Their broad specificity relies on evolutionarily conserved pattern recognition domains which interact with a variety of microbial, apoptotic and modified self ligands. Studies of immune functions of SR-expressing cells require the identification of interaction partners for SRs. We have developed an ELISA-based method which allows for large-scale high-throughput screening of complex mixtures. The assay successfully identified bacterial and plasma ligands for macrophage scavenger receptor A and can be adapted to screen for novel exogenous or endogenous ligands for any immune receptor of interest.",

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doi = "10.1007/978-1-61779-139-0_3",

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AU - Neyen, Claudine

AU - Pluddemann, Annette

AU - Gordon, Siamon

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AB - Scavenger receptors (SRs) are structurally diverse but functionally related innate immune receptors involved in defence and clearance mechanisms. Their broad specificity relies on evolutionarily conserved pattern recognition domains which interact with a variety of microbial, apoptotic and modified self ligands. Studies of immune functions of SR-expressing cells require the identification of interaction partners for SRs. We have developed an ELISA-based method which allows for large-scale high-throughput screening of complex mixtures. The assay successfully identified bacterial and plasma ligands for macrophage scavenger receptor A and can be adapted to screen for novel exogenous or endogenous ligands for any immune receptor of interest.

KW - ELISA

KW - Far Western Blot

KW - Innate immunity

KW - Interaction

KW - Ligand

KW - Macrophage

KW - Receptor

KW - Scavenger receptor

KW - Screening

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SN - 9781617791383

T3 - Methods in Molecular Biology

SP - 35

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BT - Immune Receptors

PB - Humana Press Inc.

ER -

Identification of Scavenger Receptor Ligands

Abstract

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Bibliographical note

Keywords

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