Identification of secretory gelsolin as a plasma biomarker associated with distant organ metastasis of colorectal cancer

Ming Hung Tsai, Chih Ching Wu, Pei Hua Peng, Ying Liang, Yung Chin Hsiao, Kun Yi Chien, Jeng Ting Chen, Shin Jie Lin, Rei Ping Tang, Ling Ling Hsieh*, Jau Song Yu

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

24 Scopus citations

Abstract

Colorectal cancer (CRC) is one of the most common cancers worldwide. More than half of all CRC patients will develop metastases, which represents the major cause of death for CRC patients. CRC metastases confined in other organs are potentially resectable, and patients who receive curative resections appear to have better outcomes. Thus, the early detection of metastasis in CRC patients could improve their survival rate after curative surgery. Here, we report the use of Cy-dye labeling combined with multi-dimensional fractionation and mass spectrometry as a proteomics-based approach for identifying CRC metastasis-associated biomarker(s) in plasma samples collected from three CRC patients upon diagnosis of their primary and metastatic tumors. Among the eight identified proteins, we used Western blot analysis and an in-house-developed ELISA to validate the increased plasma levels of one, secretory (plasma) gelsolin, in >80% of CRC patients with distal metastases in a larger sample cohort (32 patients). We also found a significant increase of secretory gelsolin in plasma samples of stage IV versus stages I-III CRC patients before treatment. Furthermore, immunohistochemistry showed that secretory gelsolin was highly overexpressed in CRC tissue specimens compared to adjacent normal tissues, and a cell model study showed that secretory gelsolin may help regulate CRC cell migration.

Original languageEnglish
Pages (from-to)187-200
Number of pages14
JournalJournal of Molecular Medicine
Volume90
Issue number2
DOIs
StatePublished - 02 2012

Keywords

  • Colorectal cancer
  • Metastasis
  • Migration
  • Plasma biomarker
  • Secretory gelsolin

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