Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model

Tair Long Pan; Chen Lung Lin; Chao Long Chen; Yu Chun Lin; Satoshi Gojo; Tzong Hsien Lee; Yu Hsueh Wang; Roger Lord; Chia Yun Lai; Li Weng Tsu; Hui Peng Tseng; Mei Ling Wu; Yukio Iwashita; Seigo Kitano; Kuei Chen Chiang; Takashi Hashimoto; Atsushi Sugioka; Shigeru Goto

doi:10.1016/S0966-3274(00)00024-1

Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model

Tair Long Pan, Chen Lung Lin, Chao Long Chen, Yu Chun Lin, Satoshi Gojo, Tzong Hsien Lee, Yu Hsueh Wang, Roger Lord, Chia Yun Lai, Li Weng Tsu, Hui Peng Tseng, Mei Ling Wu, Yukio Iwashita, Seigo Kitano, Kuei Chen Chiang, Takashi Hashimoto, Atsushi Sugioka, Shigeru Goto^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

12 Scopus citations

Abstract

A tryptophan catabolizer, indoleamine 2,3-dioxygenase (IDO) is highly expressed in the placenta and plays an essential role in maternal tolerance. Recent data have shown that the administration of an IDO inhibitor blocked not only maternal tolerance but also liver allograft tolerance. However, little is known about the induction of IDO in liver allografts, although a gene specific for tryptophan 2,3-dioxygenase (TDO) is believed to be expressed in the liver. In the present study, we investigated whether IDO is induced in liver allografts. Synthetic oligonucleotide primers based on the mouse IDO cDNA sequence were used to amplify RNA derived from livers of donor, syngeneic or allogeneic OLT rats. RNA encoding IDO was induced in the rat allogeneic liver after orthotopic liver transplantation (OLT), but not in syngeneic OLT. The rat nucleotide sequence of the RT-PCR products obtained from OLT livers revealed identities of 89% homology to the mouse IDO and of 68% to the human IDO. This study demonstrated the presence of RNA encoding IDO in allogeneic OLT livers, which may be involved in the immune response after liver transplantation.

Original language	English
Pages (from-to)	189-194
Number of pages	6
Journal	Transplant Immunology
Volume	8
Issue number	3
DOIs	https://doi.org/10.1016/S0966-3274(00)00024-1
State	Published - 2000
Externally published	Yes

Keywords

Indoleamine 2,3-dioxygenase
Liver transplantation
Orthotopic liver transplantation (OLT)
Rat
Rejection
Reverse transcription (RT)-PCR
Tolerance
Tryptophan

Access to Document

10.1016/S0966-3274(00)00024-1

Cite this

Pan, T. L., Lin, C. L., Chen, C. L., Lin, Y. C., Gojo, S., Lee, T. H., Wang, Y. H., Lord, R., Lai, C. Y., Tsu, L. W., Tseng, H. P., Wu, M. L., Iwashita, Y., Kitano, S., Chiang, K. C., Hashimoto, T., Sugioka, A., & Goto, S. (2000). Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model. Transplant Immunology, 8(3), 189-194. https://doi.org/10.1016/S0966-3274(00)00024-1

@article{cb12a7bf71e4440d8cf545cd3ac4906e,

title = "Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model",

abstract = "A tryptophan catabolizer, indoleamine 2,3-dioxygenase (IDO) is highly expressed in the placenta and plays an essential role in maternal tolerance. Recent data have shown that the administration of an IDO inhibitor blocked not only maternal tolerance but also liver allograft tolerance. However, little is known about the induction of IDO in liver allografts, although a gene specific for tryptophan 2,3-dioxygenase (TDO) is believed to be expressed in the liver. In the present study, we investigated whether IDO is induced in liver allografts. Synthetic oligonucleotide primers based on the mouse IDO cDNA sequence were used to amplify RNA derived from livers of donor, syngeneic or allogeneic OLT rats. RNA encoding IDO was induced in the rat allogeneic liver after orthotopic liver transplantation (OLT), but not in syngeneic OLT. The rat nucleotide sequence of the RT-PCR products obtained from OLT livers revealed identities of 89% homology to the mouse IDO and of 68% to the human IDO. This study demonstrated the presence of RNA encoding IDO in allogeneic OLT livers, which may be involved in the immune response after liver transplantation.",

keywords = "Indoleamine 2,3-dioxygenase, Liver transplantation, Orthotopic liver transplantation (OLT), Rat, Rejection, Reverse transcription (RT)-PCR, Tolerance, Tryptophan",

author = "Pan, {Tair Long} and Lin, {Chen Lung} and Chen, {Chao Long} and Lin, {Yu Chun} and Satoshi Gojo and Lee, {Tzong Hsien} and Wang, {Yu Hsueh} and Roger Lord and Lai, {Chia Yun} and Tsu, {Li Weng} and Tseng, {Hui Peng} and Wu, {Mei Ling} and Yukio Iwashita and Seigo Kitano and Chiang, {Kuei Chen} and Takashi Hashimoto and Atsushi Sugioka and Shigeru Goto",

year = "2000",

doi = "10.1016/S0966-3274(00)00024-1",

language = "英语",

volume = "8",

pages = "189--194",

journal = "Transplant Immunology",

issn = "0966-3274",

publisher = "Elsevier B.V.",

number = "3",

}

Pan, TL, Lin, CL, Chen, CL, Lin, YC, Gojo, S, Lee, TH, Wang, YH, Lord, R, Lai, CY, Tsu, LW, Tseng, HP, Wu, ML, Iwashita, Y, Kitano, S, Chiang, KC, Hashimoto, T, Sugioka, A & Goto, S 2000, 'Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model', Transplant Immunology, vol. 8, no. 3, pp. 189-194. https://doi.org/10.1016/S0966-3274(00)00024-1

TY - JOUR

T1 - Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model

AU - Pan, Tair Long

AU - Lin, Chen Lung

AU - Chen, Chao Long

AU - Lin, Yu Chun

AU - Gojo, Satoshi

AU - Lee, Tzong Hsien

AU - Wang, Yu Hsueh

AU - Lord, Roger

AU - Lai, Chia Yun

AU - Tsu, Li Weng

AU - Tseng, Hui Peng

AU - Wu, Mei Ling

AU - Iwashita, Yukio

AU - Kitano, Seigo

AU - Chiang, Kuei Chen

AU - Hashimoto, Takashi

AU - Sugioka, Atsushi

AU - Goto, Shigeru

PY - 2000

Y1 - 2000

N2 - A tryptophan catabolizer, indoleamine 2,3-dioxygenase (IDO) is highly expressed in the placenta and plays an essential role in maternal tolerance. Recent data have shown that the administration of an IDO inhibitor blocked not only maternal tolerance but also liver allograft tolerance. However, little is known about the induction of IDO in liver allografts, although a gene specific for tryptophan 2,3-dioxygenase (TDO) is believed to be expressed in the liver. In the present study, we investigated whether IDO is induced in liver allografts. Synthetic oligonucleotide primers based on the mouse IDO cDNA sequence were used to amplify RNA derived from livers of donor, syngeneic or allogeneic OLT rats. RNA encoding IDO was induced in the rat allogeneic liver after orthotopic liver transplantation (OLT), but not in syngeneic OLT. The rat nucleotide sequence of the RT-PCR products obtained from OLT livers revealed identities of 89% homology to the mouse IDO and of 68% to the human IDO. This study demonstrated the presence of RNA encoding IDO in allogeneic OLT livers, which may be involved in the immune response after liver transplantation.

AB - A tryptophan catabolizer, indoleamine 2,3-dioxygenase (IDO) is highly expressed in the placenta and plays an essential role in maternal tolerance. Recent data have shown that the administration of an IDO inhibitor blocked not only maternal tolerance but also liver allograft tolerance. However, little is known about the induction of IDO in liver allografts, although a gene specific for tryptophan 2,3-dioxygenase (TDO) is believed to be expressed in the liver. In the present study, we investigated whether IDO is induced in liver allografts. Synthetic oligonucleotide primers based on the mouse IDO cDNA sequence were used to amplify RNA derived from livers of donor, syngeneic or allogeneic OLT rats. RNA encoding IDO was induced in the rat allogeneic liver after orthotopic liver transplantation (OLT), but not in syngeneic OLT. The rat nucleotide sequence of the RT-PCR products obtained from OLT livers revealed identities of 89% homology to the mouse IDO and of 68% to the human IDO. This study demonstrated the presence of RNA encoding IDO in allogeneic OLT livers, which may be involved in the immune response after liver transplantation.

KW - Indoleamine 2,3-dioxygenase

KW - Liver transplantation

KW - Orthotopic liver transplantation (OLT)

KW - Rat

KW - Rejection

KW - Reverse transcription (RT)-PCR

KW - Tolerance

KW - Tryptophan

UR - http://www.scopus.com/inward/record.url?scp=0034534968&partnerID=8YFLogxK

U2 - 10.1016/S0966-3274(00)00024-1

DO - 10.1016/S0966-3274(00)00024-1

M3 - 文章

C2 - 11147699

AN - SCOPUS:0034534968

SN - 0966-3274

VL - 8

SP - 189

EP - 194

JO - Transplant Immunology

JF - Transplant Immunology

IS - 3

ER -

Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this