Identification of two down-regulated genes in rat liver allografts by mRNA differential display

Yu Chun Lin; S. Goto; Tair Long Pan; Yi Ren Hong; Chen Lung Lin; Roger Lord; Kuei Chen Chiang; Chia Yun Lai; Hui Peng Tseng; Li Wen Hsu; Sachio Iwashita; Seigo Kitano; Chao Long Chen

doi:10.1007/s001470100311

Identification of two down-regulated genes in rat liver allografts by mRNA differential display

Yu Chun Lin, S. Goto^*, Tair Long Pan, Yi Ren Hong, Chen Lung Lin, Roger Lord, Kuei Chen Chiang, Chia Yun Lai, Hui Peng Tseng, Li Wen Hsu, Sachio Iwashita, Seigo Kitano, Chao Long Chen

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

3 Scopus citations

Abstract

Total RNA differential display (DD) using random primers was performed for rat orthotopic liver transplantation (OLT) models. DA (RT1^a) donor livers were transplanted into DA, PVG (RT1^c), and LEW (RT1^l) recipients: (1) syngeneic OLT (DA-DA): no rejection occurs; (2) allogeneic OLT (DA-PVG): rejection occurs, but is naturally overcome without immuno-suppression; (3) allogeneic OLT (DA-LEW): animals die of acute rejection within 14 days. cDNA was isolated from selected bands, re-amplified for sequencing, and confirmed by Northern blots. Two down-regulated genes were observed in day-7 allogeneic OLT livers (DA-PVG, DA-LEW), while they were consistently expressed in day-7 syngeneic OLT (DA-DA) livers. These two genes were identified as α-glutathione sulfotransferase (α-GST) Ya gene and estrogen sulfotransferase (EST), respectively. Northern blots confirmed that their expression was down-regulated in OLT (DA-PVG) livers on days 7-26 and gradually restored. The mRNA expression of GST and EST may be good markers to predict rejection or induction of tolerance.

Original language	English
Pages (from-to)	153-158
Number of pages	6
Journal	Transplant International
Volume	14
Issue number	3
DOIs	https://doi.org/10.1007/s001470100311
State	Published - 2001
Externally published	Yes

Keywords

Estrogen sulfotransferase
Glutathione sulfotransferase
Liver transplantation
Rat
Tolerance

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title = "Identification of two down-regulated genes in rat liver allografts by mRNA differential display",

abstract = "Total RNA differential display (DD) using random primers was performed for rat orthotopic liver transplantation (OLT) models. DA (RT1a) donor livers were transplanted into DA, PVG (RT1c), and LEW (RT1l) recipients: (1) syngeneic OLT (DA-DA): no rejection occurs; (2) allogeneic OLT (DA-PVG): rejection occurs, but is naturally overcome without immuno-suppression; (3) allogeneic OLT (DA-LEW): animals die of acute rejection within 14 days. cDNA was isolated from selected bands, re-amplified for sequencing, and confirmed by Northern blots. Two down-regulated genes were observed in day-7 allogeneic OLT livers (DA-PVG, DA-LEW), while they were consistently expressed in day-7 syngeneic OLT (DA-DA) livers. These two genes were identified as α-glutathione sulfotransferase (α-GST) Ya gene and estrogen sulfotransferase (EST), respectively. Northern blots confirmed that their expression was down-regulated in OLT (DA-PVG) livers on days 7-26 and gradually restored. The mRNA expression of GST and EST may be good markers to predict rejection or induction of tolerance.",

keywords = "Estrogen sulfotransferase, Glutathione sulfotransferase, Liver transplantation, Rat, Tolerance",

author = "Lin, {Yu Chun} and S. Goto and Pan, {Tair Long} and Hong, {Yi Ren} and Lin, {Chen Lung} and Roger Lord and Chiang, {Kuei Chen} and Lai, {Chia Yun} and Tseng, {Hui Peng} and Hsu, {Li Wen} and Sachio Iwashita and Seigo Kitano and Chen, {Chao Long}",

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language = "英语",

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journal = "Transplant International",

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TY - JOUR

T1 - Identification of two down-regulated genes in rat liver allografts by mRNA differential display

AU - Lin, Yu Chun

AU - Goto, S.

AU - Pan, Tair Long

AU - Hong, Yi Ren

AU - Lin, Chen Lung

AU - Lord, Roger

AU - Chiang, Kuei Chen

AU - Lai, Chia Yun

AU - Tseng, Hui Peng

AU - Hsu, Li Wen

AU - Iwashita, Sachio

AU - Kitano, Seigo

AU - Chen, Chao Long

PY - 2001

Y1 - 2001

N2 - Total RNA differential display (DD) using random primers was performed for rat orthotopic liver transplantation (OLT) models. DA (RT1a) donor livers were transplanted into DA, PVG (RT1c), and LEW (RT1l) recipients: (1) syngeneic OLT (DA-DA): no rejection occurs; (2) allogeneic OLT (DA-PVG): rejection occurs, but is naturally overcome without immuno-suppression; (3) allogeneic OLT (DA-LEW): animals die of acute rejection within 14 days. cDNA was isolated from selected bands, re-amplified for sequencing, and confirmed by Northern blots. Two down-regulated genes were observed in day-7 allogeneic OLT livers (DA-PVG, DA-LEW), while they were consistently expressed in day-7 syngeneic OLT (DA-DA) livers. These two genes were identified as α-glutathione sulfotransferase (α-GST) Ya gene and estrogen sulfotransferase (EST), respectively. Northern blots confirmed that their expression was down-regulated in OLT (DA-PVG) livers on days 7-26 and gradually restored. The mRNA expression of GST and EST may be good markers to predict rejection or induction of tolerance.

AB - Total RNA differential display (DD) using random primers was performed for rat orthotopic liver transplantation (OLT) models. DA (RT1a) donor livers were transplanted into DA, PVG (RT1c), and LEW (RT1l) recipients: (1) syngeneic OLT (DA-DA): no rejection occurs; (2) allogeneic OLT (DA-PVG): rejection occurs, but is naturally overcome without immuno-suppression; (3) allogeneic OLT (DA-LEW): animals die of acute rejection within 14 days. cDNA was isolated from selected bands, re-amplified for sequencing, and confirmed by Northern blots. Two down-regulated genes were observed in day-7 allogeneic OLT livers (DA-PVG, DA-LEW), while they were consistently expressed in day-7 syngeneic OLT (DA-DA) livers. These two genes were identified as α-glutathione sulfotransferase (α-GST) Ya gene and estrogen sulfotransferase (EST), respectively. Northern blots confirmed that their expression was down-regulated in OLT (DA-PVG) livers on days 7-26 and gradually restored. The mRNA expression of GST and EST may be good markers to predict rejection or induction of tolerance.

KW - Estrogen sulfotransferase

KW - Glutathione sulfotransferase

KW - Liver transplantation

KW - Rat

KW - Tolerance

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U2 - 10.1007/s001470100311

DO - 10.1007/s001470100311

M3 - 文章

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SN - 0934-0874

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SP - 153

EP - 158

JO - Transplant International

JF - Transplant International

IS - 3

ER -

Identification of two down-regulated genes in rat liver allografts by mRNA differential display

Abstract

Keywords

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