Impact of rosuvastatin treatment on reduction of thrombus burden in rat acute inferior vena cava stenosis

Kun Chen Lin; Hung I. Lu; Yung Lung Chen; Tzu Hsien Tsai; Li Teh Chang; Steve Leu; Shu Yuan Hsu; Cheuk Kwan Sun; Han Tan Chai; Hsueh Wen Chang; Chia Lo Chang; Hong Hwa Chen; Hon Kan Yip

doi:10.1186/s12950-014-0027-2

Impact of rosuvastatin treatment on reduction of thrombus burden in rat acute inferior vena cava stenosis

Kun Chen Lin, Hung I. Lu, Yung Lung Chen, Tzu Hsien Tsai, Li Teh Chang, Steve Leu, Shu Yuan Hsu, Cheuk Kwan Sun, Han Tan Chai, Hsueh Wen Chang, Chia Lo Chang, Hong Hwa Chen, Hon Kan Yip^*

^*Corresponding author for this work

Department of Anatomy

Research output: Contribution to journal › Journal Article › peer-review

1 Scopus citations

Abstract

Background: This study tested the hypothesis that rosuvastatin reduces thrombus burden through inhibiting inflammation and suppressing reactive oxygen species (ROS) generation in an inferior vena cava stenosis (IVC_ST)-induced deep vein thrombosis (DVT) rat model.

Methods. 12-week-old male Sprague-Dawley rats (n = 24) were equally divided into sham control (group 1: laparotomy only), IVC_ST (group 2: IVC stenosis), and IVC_ST + rosuvastatin (20 mg/kg/day, orally after induction of IVC stenosis) (group 3). IVC diameter was measured by days 0 and 14 and the right hindlimb thickness was measured by day 0, 7, and 14 prior to scarifying the animals.

Results: The results showed significantly increased IVC diameter and hindlimb thickness in group 2 than in groups 1 and 3, and significantly increased in group 3 than in group 1 by day 14 after the procedure (all p < 0.001). Additionally, WBC count and prevalence of helper T cells, cytotoxic T cells, regulatory T cells, and early and late apoptotic mononuclear cells (MNCs) in circulation were significantly higher in group 2 than in group 1, and were significantly suppressed in group 3 after treatment (all p < 0.001). Furthermore, inflammation at cellular (CD68+ cells) and protein (MMP-9, TNF-α) levels, oxidative stress (oxidized protein) and reactive oxygen species (NOX-1, NOX-2) in IVC also showed similar changes as those of immune cells in circulation among the three groups (all p < 0.01).

Conclusion: Rosuvastatin treatment significantly reduced IVC thrombus burden through inhibiting inflammatory response and oxidative stress in a rodent model of DVT.

Original language	English
Article number	27
Journal	Journal of Inflammation (United Kingdom)
Volume	11
Issue number	1
DOIs	https://doi.org/10.1186/s12950-014-0027-2
State	Published - 12 09 2014

Bibliographical note

Publisher Copyright:
© 2014 Lin et al.

Access to Document

10.1186/s12950-014-0027-2

Cite this

Lin, K. C., Lu, H. I., Chen, Y. L., Tsai, T. H., Chang, L. T., Leu, S., Hsu, S. Y., Sun, C. K., Chai, H. T., Chang, H. W., Chang, C. L., Chen, H. H., & Yip, H. K. (2014). Impact of rosuvastatin treatment on reduction of thrombus burden in rat acute inferior vena cava stenosis. Journal of Inflammation (United Kingdom), 11(1), Article 27. https://doi.org/10.1186/s12950-014-0027-2

@article{ff0848008d1c4984ad430bde649c37d8,

title = "Impact of rosuvastatin treatment on reduction of thrombus burden in rat acute inferior vena cava stenosis",

abstract = "Background: This study tested the hypothesis that rosuvastatin reduces thrombus burden through inhibiting inflammation and suppressing reactive oxygen species (ROS) generation in an inferior vena cava stenosis (IVCST)-induced deep vein thrombosis (DVT) rat model.Methods. 12-week-old male Sprague-Dawley rats (n = 24) were equally divided into sham control (group 1: laparotomy only), IVCST (group 2: IVC stenosis), and IVCST + rosuvastatin (20 mg/kg/day, orally after induction of IVC stenosis) (group 3). IVC diameter was measured by days 0 and 14 and the right hindlimb thickness was measured by day 0, 7, and 14 prior to scarifying the animals.Results: The results showed significantly increased IVC diameter and hindlimb thickness in group 2 than in groups 1 and 3, and significantly increased in group 3 than in group 1 by day 14 after the procedure (all p < 0.001). Additionally, WBC count and prevalence of helper T cells, cytotoxic T cells, regulatory T cells, and early and late apoptotic mononuclear cells (MNCs) in circulation were significantly higher in group 2 than in group 1, and were significantly suppressed in group 3 after treatment (all p < 0.001). Furthermore, inflammation at cellular (CD68+ cells) and protein (MMP-9, TNF-α) levels, oxidative stress (oxidized protein) and reactive oxygen species (NOX-1, NOX-2) in IVC also showed similar changes as those of immune cells in circulation among the three groups (all p < 0.01).Conclusion: Rosuvastatin treatment significantly reduced IVC thrombus burden through inhibiting inflammatory response and oxidative stress in a rodent model of DVT.",

author = "Lin, {Kun Chen} and Lu, {Hung I.} and Chen, {Yung Lung} and Tsai, {Tzu Hsien} and Chang, {Li Teh} and Steve Leu and Hsu, {Shu Yuan} and Sun, {Cheuk Kwan} and Chai, {Han Tan} and Chang, {Hsueh Wen} and Chang, {Chia Lo} and Chen, {Hong Hwa} and Yip, {Hon Kan}",

note = "Publisher Copyright: {\textcopyright} 2014 Lin et al.",

year = "2014",

month = sep,

day = "12",

doi = "10.1186/s12950-014-0027-2",

language = "英语",

volume = "11",

journal = "Journal of Inflammation (United Kingdom)",

issn = "1476-9255",

publisher = "BioMed Central Ltd",

number = "1",

}

TY - JOUR

T1 - Impact of rosuvastatin treatment on reduction of thrombus burden in rat acute inferior vena cava stenosis

AU - Lin, Kun Chen

AU - Lu, Hung I.

AU - Chen, Yung Lung

AU - Tsai, Tzu Hsien

AU - Chang, Li Teh

AU - Leu, Steve

AU - Hsu, Shu Yuan

AU - Sun, Cheuk Kwan

AU - Chai, Han Tan

AU - Chang, Hsueh Wen

AU - Chang, Chia Lo

AU - Chen, Hong Hwa

AU - Yip, Hon Kan

PY - 2014/9/12

Y1 - 2014/9/12

N2 - Background: This study tested the hypothesis that rosuvastatin reduces thrombus burden through inhibiting inflammation and suppressing reactive oxygen species (ROS) generation in an inferior vena cava stenosis (IVCST)-induced deep vein thrombosis (DVT) rat model.Methods. 12-week-old male Sprague-Dawley rats (n = 24) were equally divided into sham control (group 1: laparotomy only), IVCST (group 2: IVC stenosis), and IVCST + rosuvastatin (20 mg/kg/day, orally after induction of IVC stenosis) (group 3). IVC diameter was measured by days 0 and 14 and the right hindlimb thickness was measured by day 0, 7, and 14 prior to scarifying the animals.Results: The results showed significantly increased IVC diameter and hindlimb thickness in group 2 than in groups 1 and 3, and significantly increased in group 3 than in group 1 by day 14 after the procedure (all p < 0.001). Additionally, WBC count and prevalence of helper T cells, cytotoxic T cells, regulatory T cells, and early and late apoptotic mononuclear cells (MNCs) in circulation were significantly higher in group 2 than in group 1, and were significantly suppressed in group 3 after treatment (all p < 0.001). Furthermore, inflammation at cellular (CD68+ cells) and protein (MMP-9, TNF-α) levels, oxidative stress (oxidized protein) and reactive oxygen species (NOX-1, NOX-2) in IVC also showed similar changes as those of immune cells in circulation among the three groups (all p < 0.01).Conclusion: Rosuvastatin treatment significantly reduced IVC thrombus burden through inhibiting inflammatory response and oxidative stress in a rodent model of DVT.

AB - Background: This study tested the hypothesis that rosuvastatin reduces thrombus burden through inhibiting inflammation and suppressing reactive oxygen species (ROS) generation in an inferior vena cava stenosis (IVCST)-induced deep vein thrombosis (DVT) rat model.Methods. 12-week-old male Sprague-Dawley rats (n = 24) were equally divided into sham control (group 1: laparotomy only), IVCST (group 2: IVC stenosis), and IVCST + rosuvastatin (20 mg/kg/day, orally after induction of IVC stenosis) (group 3). IVC diameter was measured by days 0 and 14 and the right hindlimb thickness was measured by day 0, 7, and 14 prior to scarifying the animals.Results: The results showed significantly increased IVC diameter and hindlimb thickness in group 2 than in groups 1 and 3, and significantly increased in group 3 than in group 1 by day 14 after the procedure (all p < 0.001). Additionally, WBC count and prevalence of helper T cells, cytotoxic T cells, regulatory T cells, and early and late apoptotic mononuclear cells (MNCs) in circulation were significantly higher in group 2 than in group 1, and were significantly suppressed in group 3 after treatment (all p < 0.001). Furthermore, inflammation at cellular (CD68+ cells) and protein (MMP-9, TNF-α) levels, oxidative stress (oxidized protein) and reactive oxygen species (NOX-1, NOX-2) in IVC also showed similar changes as those of immune cells in circulation among the three groups (all p < 0.01).Conclusion: Rosuvastatin treatment significantly reduced IVC thrombus burden through inhibiting inflammatory response and oxidative stress in a rodent model of DVT.

UR - http://www.scopus.com/inward/record.url?scp=84907685938&partnerID=8YFLogxK

U2 - 10.1186/s12950-014-0027-2

DO - 10.1186/s12950-014-0027-2

M3 - 文章

AN - SCOPUS:84907685938

SN - 1476-9255

VL - 11

JO - Journal of Inflammation (United Kingdom)

JF - Journal of Inflammation (United Kingdom)

IS - 1

M1 - 27

ER -

Impact of rosuvastatin treatment on reduction of thrombus burden in rat acute inferior vena cava stenosis

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this