Impaired phagocytic mechanism in annexin 1 null macrophages

Simon Yona, Sigrid E.M. Heinsbroek, Leanne Peiser, Siamon Gordon, Mauro Perretti, Roderick J. Flower*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

48 Scopus citations

Abstract

1 The role of the anti-inflammatory protein annexin-A1 (Anx-A1) in the phagocytic process has been investigated using a murine bone marrow culture-derived macrophage model from Anx-A1 +/+ and Anx-A1 -/- mice. 2 Macrophages prepared from Anx-A1 -/- mice exhibited a reduced ingestion of zymosan, Neisseria meningitidis or sheep red blood cells, when compared to Anx-A1 +/+ cells and in the case of zymosan this effect was also mirrored by a reduced clearance in vivo when particles were injected into the peritoneal cavity of Anx-A1 -/- mice. 3 The ablation of the Anx-A1 gene did not cause any apparent cytoskeletal defects associated with particle ingestion but the cell surface expression of the key adhesion molecule CD11b was depressed in the Anx-A1 -/- cells providing a possible explanation for the attenuated phagocytic potential of these cells. 4 The production of the cytokines TNFα and IL-6 was increased in Anx-A1 -/- macrophages following phagocytosis of all types of particle.

Original languageEnglish
Pages (from-to)469-477
Number of pages9
JournalBritish Journal of Pharmacology
Volume148
Issue number4
DOIs
StatePublished - 08 06 2006
Externally publishedYes

Keywords

  • Annexin A1
  • CD11b
  • Inflammation and cytokines
  • N. meningitidis
  • Zymosan

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