Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Jheng Syong Wu; Yung Chin Chung; Jun Jei Chien; Chien Chou

doi:10.1117/1.JBO.23.1.010502

Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Jheng Syong Wu, Yung Chin Chung, Jun Jei Chien, Chien Chou^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

4 Scopus citations

Abstract

A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

Original language	English
Article number	010502
Journal	Journal of Biomedical Optics
Volume	23
Issue number	1
DOIs	https://doi.org/10.1117/1.JBO.23.1.010502
State	Published - 01 2018
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).

Keywords

aberrations
confocal fluorescence microscopy
optical heterodyne

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10.1117/1.JBO.23.1.010502

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abstract = "A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.",

keywords = "aberrations, confocal fluorescence microscopy, optical heterodyne",

author = "Wu, {Jheng Syong} and Chung, {Yung Chin} and Chien, {Jun Jei} and Chien Chou",

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AU - Chien, Jun Jei

AU - Chou, Chien

PY - 2018/1

Y1 - 2018/1

N2 - A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

AB - A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

KW - aberrations

KW - confocal fluorescence microscopy

KW - optical heterodyne

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DO - 10.1117/1.JBO.23.1.010502

M3 - 文章

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AN - SCOPUS:85040829620

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ER -

Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Abstract

Bibliographical note

Keywords

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