Abstract
A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.
| Original language | English |
|---|---|
| Article number | 010502 |
| Journal | Journal of Biomedical Optics |
| Volume | 23 |
| Issue number | 1 |
| DOIs | |
| State | Published - 01 2018 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).
Keywords
- aberrations
- confocal fluorescence microscopy
- optical heterodyne
Fingerprint
Dive into the research topics of 'Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver